Clinical Comparison of Two Photosensitizers for Oral Cavity Decontamination

Abstract

Objective: In this study, we aim to compare the photodynamic inactivation (PDI) effects of two different photosensitizers (PS), Photogem^® and Natural Curcumin, irradiated with light-emitted diodes (LED) at 630 and 450 nm, respectively.

Background: The current antimicrobial mouthwash for oral hygiene has several drawbacks. In this context, PDI is an alternative technique to inactivate pathogenic microbes in mucosa and in periodontal tissue. Furthermore, there are numerous infectious diseases that may affect the oral cavity, motivating the use of PDI in dentistry.

Methods: The volunteers (n = 50) were randomize separated into five experimental groups (n = 5) for each PS: water control, PS control, light control, and two PS concentrations (25 and 100 mg/L). Each patient underwent mouthwash solution containing the PS before illumination procedure that was performed with an LED device. For microbial decontamination evaluation, the saliva was collected three times: before (T0), immediately after (T1), and 24 h after the illumination procedure (T2). After that, the difference between the colony forming units (CFU) for each volunteer was compared.

Results: The results show that regardless of PS and treatment applied, there was microbial reduction immediately after PDI, however, after 24 h only Natural Curcumin still presents a reduction. For Photogem after 24 h, the microorganism returns to the original CFU.

Conclusions: Immediately after PDI, both PS have the same efficiency, nevertheless the Natural Curcumin still has an efficacy after 24 h and also is a more viable photosensitizer. In addition, the results indicate that PDI can be a promised technique used for microbial reducing for the oral cavity.

Keywords: clinical; curcuminoids; hematoporphyrin; in vivo; photodynamic inactivation.