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. 2016 Dec;26(12):1353-1356.
doi: 10.1038/cr.2016.133. Epub 2016 Nov 11.

Structural basis for specific recognition of pre-snRNA by Gemin5

Xuhua Tang  1 , Sakshibeedu R Bharath  1 , Shunfu Piao  1 , Vanessa Qianmin Tan  1 , Matthew W Bowler  2   3 , Haiwei Song  1   4
Affiliations

Structural basis for specific recognition of pre-snRNA by Gemin5

Xuhua Tang et al. Cell Res. 2016 Dec.
No abstract available

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Figures

Figure 1
Figure 1
Specific recognition of m7GpppG cap and the Sm site of pre-snRNA by Gemin5-WD. (A) Overall structure of Gemin5-WD in complex with m7GpppG cap. WD1 and WD2 of Gemin5-WD are shown in light blue and green, respectively. m7Gppp of the cap is shown in purple. (B) Interactions of the m7GpppG cap analog with Gemin5-WD. Residues involved in the interaction are shown as sticks and labeled. Hydrogen bonds are shown as pink dashed lines. (C) Cap-binding assays were performed with wild-type (WT) Gemin5-WD and its mutants. (D) Electrostatic surface potential of Gemin5-WD complexed with Sm RNA (−7.5–7.5 kT/e). Sm RNA (nt 118-127 in pre-U4) used in the study is shown with specificity-determining nucleotides in red and those not observed in the structure in grey. (E) Interactions of Sm RNA with Gemin5-WD. (F) Fluorescence anisotropy measurements of Sm RNA (nt 118-127) binding with Gemin5-WD and its variants. (G) RNA-binding assays of Gemin5-WD in the absence or the presence of 2.5-fold excess of m7GpppG. Sm RNA (nt 118-127) and longer U4-RNA (nt 85-145 in pre-U4) are used. (H) The specificity determinants of Sm RNA for Gemin5 recognition were examined by fluorescence anisotropy.
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