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. 2016 Dec 20;7(51):85097-85108.
doi: 10.18632/oncotarget.13197.

Subsite-specific association of DEAD box RNA helicase DDX60 with the development and prognosis of oral squamous cell carcinoma

Ting-Ying Fu  1   2 , Chao-Nan Wu  3   4 , Huei-Cin Sie  1 , Jiin-Tsuey Cheng  5 , Yaoh-Shiang Lin  6 , Huei-Han Liou  7 , Yu-Kai Tseng  8   9 , Chih-Wen Shu  7 , Kuo-Wang Tsai  7   10 , Leing-Ming Yen  1 , Hui-Wen Tseng  11   12 , Ching-Jiunn Tseng  7   13   14   15 , Luo-Ping Ger  7   16 , Pei-Feng Liu  7   17
Affiliations

Subsite-specific association of DEAD box RNA helicase DDX60 with the development and prognosis of oral squamous cell carcinoma

Ting-Ying Fu et al. Oncotarget. .

Abstract

The clinical significance and biological function of DEXD/H box helicase 60 (DDX60) in oral cancer remains unknown. Herein, we evaluated the association of DDX60 expression with tumorigenesis and the prognosis of oral squamous cell carcinoma (OSCC). DDX60 expression was examined by immunohistochemistry on tissue microarray slides of 494 OSCC patients, including 180 buccal mucosal SCC (BMSCC), 241 tongue SCC (TSCC), and 73 lip SCC (LSCC) patients. DDX60 expression was significantly increased in all three subsites of OSCC compared to its expression in tumor adjacent normal tissues. However, its association with tumorigenesis was specific to the oral cavity subsite after the stratification of betel quid chewing, smoking, and drinking. Among OSCC patients, higher levels of DDX60 expression were associated with the male gender, a well-differentiated tumor, advanced stage of disease, and a large tumor size with subsite specific features. LSCC patients with high DDX60 expression levels showed shorter disease-specific survival, particularly those with moderately or poorly differentiated tumors. Additionally, TSCC or OSCC patients with high DDX60 expression showed a poor disease-free survival (DFS), particularly those with moderately or poorly differentiated tumors. Therefore, DDX60 is a novel and unfavorable biomarker for tumorigenesis and prognosis of OSCC in a subsite-specific manner.

Keywords: DDX60; oral cancer; prognosis; subsite-specific; tumorigenesis.

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Conflict of interest statement

CONFLICTS OF INTEREST

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1. IHC staining of DDX60 protein expression
A. The representative immunoreactivity intensity of DDX60 in OSCC for negative (-), weak (+), moderate (++), strong (+++) staining. B. The representative immunoreactivity of paired tumor and normal tissues in buccal mucosal, tongue, and lip subsites.
Figure 2
Figure 2. The Kaplan-Meier curves for disease-specific survival and recurrence-free survival with different levels of DDX60 expression in patients with BMSCC (A, E), TSCC (B, F), LSCC (C, G) and OSCC (D, H)
Figure 3
Figure 3. Differences in the survival curves between patients with high and low levels of DDX60 expression in LSCC (A, B for DSS; G, H for RFS), TSCC (C-F for RFS), and OSCC (I, J for RFS), stratified according to cell differentiation and the pathological stage
See this image and copyright information in PMC

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