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Comment
. 2016 Nov 10;12(11):887.
doi: 10.15252/msb.20167332.

Unlocking the chromatin code by deciphering protein-DNA interactions

Dalila Bensaddek  1 , Angus I Lamond  1
Affiliations
Comment

Unlocking the chromatin code by deciphering protein-DNA interactions

Dalila Bensaddek et al. Mol Syst Biol. .

Abstract

Characterizing the composition of protein complexes bound to different genomic loci is essential for advancing our mechanistic understanding of transcriptional regulation. In their recent study, Krijgsveld and colleagues (Rafiee et al, 2016) report ChIP‐SICAP, a powerful tool for deciphering the chromatin proteome by combining chromatin immunoprecipitation, selective isolation of chromatin‐associated proteins and mass spectrometry.

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Figures

Figure 1
Figure 1. SICAP method
Schematic representation of the ChIP‐SICAP workflow, which starts with in cellulo formaldehyde crosslinking, followed by sonication and immunoprecipitation of chromatin (ChIP), then by the selective isolation of chromatin‐associated proteins (SICAP). DNA biotinylation allows the removal of the antibody prior to SP3 processing for LC‐MS/MS and ChIP‐seq analyses.
See this image and copyright information in PMC

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References

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