doi: 10.1007/978-1-4939-6591-5_20.
Fluorescence Resonance Energy Transfer (FRET)-Based Analysis of Lipoplexes
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- PMID: 27837545
- DOI: 10.1007/978-1-4939-6591-5_20
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Fluorescence Resonance Energy Transfer (FRET)-Based Analysis of Lipoplexes
Methods Mol Biol.
2017.
Abstract
We describe three applications of FRET technique to analyze structural and thermodynamic properties of cationic lipids and their complexes with DNA: (1) Lipid mixing assay to determine the degree to which individual vesicles undergo fusion upon complex formation. (2) DNA binding assay to obtain cationic lipid-DNA binding curves for thermodynamic analysis or binding stoichiometry characterization. (3) DNA spacing assay to determine changes in DNA packing in the multilamellar lipoplex particle.
Keywords: Binding assay; Cationic lipid; DNA spacing; FRET; Lipid mixing; Lipoplex; Vesicle fusion.
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