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Review
. 2016:951:123-135.
doi: 10.1007/978-3-319-45457-3_10.

Cryopreservation of Human Pluripotent Stem Cell-Derived Cardiomyocytes: Strategies, Challenges, and Future Directions

Marcela K Preininger  1   2 , Monalisa Singh  1 , Chunhui Xu  3   4
Affiliations
Review

Cryopreservation of Human Pluripotent Stem Cell-Derived Cardiomyocytes: Strategies, Challenges, and Future Directions

Marcela K Preininger et al. Adv Exp Med Biol. 2016.

Abstract

In recent years, human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have emerged as a vital cell source for in vitro modeling of genetic cardiovascular disorders, drug screening, and in vivo cardiac regeneration research. Looking forward, the ability to efficiently cryopreserve hPSC-CMs without compromising their normal biochemical and physiologic functions will dramatically facilitate their various biomedical applications. Although working protocols for freezing, storing, and thawing hPSC-CMs have been established, the question remains as to whether they are optimal. In this chapter, we discuss our current understanding of cryopreservation appertaining to hPSC-CMs, and proffer key questions regarding the mechanical, contractile, and regenerative properties of cryopreserved hPSC-CMs.

Keywords: Beating; Cell viability; Contraction; Cryopreservation; Electrical coupling; Engraftment; Freezing; Human pluripotent stem cell-derived cardiomyocytes; Thawing; Transplantation.

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Figures

Figure 1
Figure 1
General procedure for the cryopreservation of hPSC-CMs
Figure 2
Figure 2
General procedure for thawing cryopreserved hPSC-CMs
See this image and copyright information in PMC

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