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Multicenter Study
. 2016 Oct;23(4):383-389.
doi: 10.1177/107327481602300409.

Construction and Validation of a Multi-Institutional Tissue Microarray of Invasive Ductal Carcinoma From Racially and Ethnically Diverse Populations

Edward Seijo  1 , Diana Lima, Egiebade Iriabho, Jonas Almeida, Jesus Monico, Margarita Echeverri, Sylvia Gutierrez, Idhaliz Flores, Ji-Hyun Lee, Kate Fisher, William E Grizzle, Gabriel L Sica, Charles Butler, Chindo Hicks, Cathy D Meade, Stephen Olufemi Sodeke, Krzysztof Moroz, Domenico Coppola, Teresita Munoz-Antonia
Affiliations
Multicenter Study

Construction and Validation of a Multi-Institutional Tissue Microarray of Invasive Ductal Carcinoma From Racially and Ethnically Diverse Populations

Edward Seijo et al. Cancer Control. 2016 Oct.

Abstract

Background: The scarcity of tissues from racial and ethnic minorities at biobanks poses a scientific constraint to research addressing health disparities in minority populations.

Methods: To address this gap, the Minority Biospecimen/Biobanking Geographic Management Program for region 3 (BMaP-3) established a working infrastructure for a "biobanking" hub in the southeastern United States and Puerto Rico. Herein we describe the steps taken to build this infrastructure, evaluate the feasibility of collecting formalin-fixed, paraffin-embedded tissue blocks and associated data from a single cancer type (breast), and create a web-based database and tissue microarrays (TMAs).

Results: Cancer registry data from 6 partner institutions were collected, representing 12,408 entries from 8,279 unique patients with breast cancer (years 2001-2011). Data were harmonized and merged, and deidentified information was made available online. A TMA was constructed from formalin-fixed, paraffin-embedded samples of invasive ductal carcinoma (IDC) representing 427 patients with breast cancer (147 African Americans, 168 Hispanics, and 112 non-Hispanic whites) and was annotated according to biomarker status and race/ethnicity. Biomarker analysis of the TMA was consistent with the literature.

Conclusions: Contributions from participating institutions have facilitated a robust research tool. TMAs of IDC have now been released for 5 projects at 5 different institutions.

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Figures

Fig 1
Fig 1. Results of breast cancer researcher survey
A multi-institutional, Web-based poll was distributed to investigators in region 3 (currently region 2) institutions to assess the research interest regarding different kinds of breast TMAs. Survey responses were anonymous. As can be seen, the greatest need was for a TMA based on biomarker status between different racial/ethnic groups. TMA = tissue microarray.
Fig 2
Fig 2. Sample representation of immunostaining according to biomarker status
TMA slides were immunostained with anti–estrogen receptor, anti–progesterone receptor, and anti–erb-b2 receptor tyrosine kinase 2 antibodies. TMA = tissue microarray.
See this image and copyright information in PMC

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