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. 2016 Nov 14;13(1):185.
doi: 10.1186/s12985-016-0597-0.

Immunogenicity of porcine circovirus type 2 nucleic acid vaccine containing CpG motif for mice

Jun Li  1 , Jiang Yu  1 , Shaojian Xu  1 , Jianli Shi  1 , Shengnan Xu  1 , Xiaoyan Wu  1 , Fang Fu  2 , Zhe Peng  1 , Lingling Zhang  1 , Shuxuan Zheng  1 , Xiaoyuan Yuan  1 , Xiaoyan Cong  1 , Wenbo Sun  1 , Kaihui Cheng  1 , Yijun Du  1 , Jiaqiang Wu  1 , Jinbao Wang  3
Affiliations

Immunogenicity of porcine circovirus type 2 nucleic acid vaccine containing CpG motif for mice

Jun Li et al. Virol J. .

Abstract

Background: This study aimed at reseaching the immune effect of porcine circovirus type 2 (PCV2) DNA vaccine containing CpG motif on mice.

Methods: A total of 40 6-week-old female BALB/c mice were randomly divided into four groups which were immunized by 18CpG-pVAX1-ORF2, pVAX1-ORF2, pVAX1 and PBS, respectively, and immunized again 2 weeks later. All mice were challenged with 0.2 mL PCV2 cells virulent strain SD (106.0 TCID50/mL) after 4 weeks. Average daily gain, blood antibody levels, microscopic changes and viremia were detected to estimate the effect of DNA vaccine.

Results and discussion: The results showed that compared to those of the control mice, groups immunized with pVAX1-ORF2 and 18CpG-pVAX1-ORF2 could induce PCV2-specific antibodies. The PCV2-specific antibodies level of 18 CpG-pVAX1-ORF2 groups was higher significantly than other groups and decreased slowly along with time. There was no distinct pathological damage and viremia occurring in mice that inoculated with CpG motif DNA vaccines. The results demonstrated that the DNA vaccine containing 18 CpG could build up resistibility immunity and reduce immune organ damage on mice.

Keywords: CpG motifs; Immunogenicity; Mice; Nucleic acid vaccine; Porcine circovirus type 2.

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Figures

Fig. 1
Fig. 1
Serum antibody level after immunization in mice
Fig. 2
Fig. 2
Histopathological changes in the lungs (A-D), kidney (E-H) and liver (I-L) of mice infected by PCV2 (HE). Mice of group I (PBS) showed alveolar walls were thick and inflammatory cells were in the lung bronchia (a). Mice of group II (pVAX1) showed interstitial pneumonia, and congestion was observed in alveolar interlobular interval and infiltration with inflammatory cells (b). A few of inflammatory cells were observed in alveoli of group III (pVAX1-ORF2) (c). Wider congestion in the glomeruli was observed in group I-III, and degenerative necrosis in kidney tubules were observed in group I and II (e, f). The typical or slight vacuoles granular degeneration was observed in hepatocyte of group I or III, respectively (i, k). The hepatocyte of group II showed slight atrophy (j). No obvious unusual changes were observed in lungs, liver and kidney of group IV (d, h, l). The scale bar is 100 mm
Fig. 3
Fig. 3
The distribution of virus in different organizations of different periods
See this image and copyright information in PMC

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