Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Jun;4(2):105-114.

Isolation and molecular characterization of partial FSH and LH receptor genes in Arabian camels (Camelus dromedarius)

Saber Jelokhani-Niaraki  1 , Mojtaba Tahmoorespur  1 , Morteza Bitaraf-Sani  1
Affiliations

Isolation and molecular characterization of partial FSH and LH receptor genes in Arabian camels (Camelus dromedarius)

Saber Jelokhani-Niaraki et al. Mol Biol Res Commun. 2015 Jun.

Abstract

Very little is known about LHR and FSHR genes of domestic dromedary camels. The main objective of this study was to determine and analyze partial genomic regions of FSHR and LHR genes in dromedary camels for the first time. To this end, a total of50 DNA samples belonging to dromedary camels raised in Iran were sent for sequencing (25 samples of each gene). We compared the nucleotide sequences of Camelus dromedarius with corresponding sequences of previously published FSHR and LHR genes in bactrian camels and other species. According to the data, the same nucleotide variation was identified in both regions of the two camel species. The alignment of deduced protein sequences of the two different species revealed an amino acid variation at the FSHR region. No evidence of amino acid variation was observed, however, in LHR sequences. Phylogenetic analysis indicated that both camel species had a close relationship and clustered together in a separate branch. This was further confirmed by genetic distance values illustrating significant sequence identity between Camelus dromedarius and Camelusbactrianus. Interestingly, sequence comparisons revealed heterozygote patterns in FSHR sequences isolated from dromedary camels of Iran. In comparison to other species, this camel contains three amino acid substitutions at 5, 67, and 105 positions in the FSHR coding region. These positions are found exclusively in camels and can be considered as species specific. The results of our study can be used for hormone functionality research (FSHR and LHR) as well as reproduction-linked polymorphisms and breeding programs.

Keywords: Camel; FSHR; LHR; Sequence.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Alignment of partial amino acid sequences of FSHR (A) and LHR (B) region. Specific residues are marked with a red box. Dot (.) indicates sequence identity
Figure 2
Figure 2
Phylogenetic tree constructed on the basis of partial sequences of FSHR (A) and LHR (B) region
Figure 3
Figure 3
(A) The heterozygote and homozygote positions on FSHR sequences in examined dromedary camels of Iran. The letters F and R are abbreviated for forward and reverse sequence. (B) The position of single nucleotide polymorphism (SNP) found between two species Bactrian and Arabian camel in LHR region
See this image and copyright information in PMC

References

    1. Boime I, Ben-Menahem D. Glycoprotein hormone structure-function and analog design. Recent Prog Horm Res. 1999;54:271–288. - PubMed
    1. McFarland KC, Sprengel R, Phillips HS, Kohler M, Rosemblit N, Nikolics K, Segaloff DL, Seeburg PH. Lutropin-choriogonadotropin receptor: an unusual member of the G protein-coupled receptor family. Science. 1989;245:494–499. - PubMed
    1. Parmentier M, Libert F, Maenhaut C, Lefort A, Gerard C, Perret J, Van Sande J, Dumont JE, Vassart G. Molecular cloning of the thyrotropin receptor. Science. 1989;246:1620–1622. - PubMed
    1. Ni Y, Zhou Y, Lu L, Grossmann R, Zhao R. Developmental changes of FSH-R, LH-R, ER-beta and GnRH-I expression in the ovary of prepubertal ducks (Anas platyrhynchos) Anim Reprod Sci. 2007;100:318–328. - PubMed
    1. Schwartz H, Dioli M. The One-Humped Camel in Eastern Africa: A Pictorial guide to diseases, health care and management. Margraf Scientific Book Berlin; 1992.

LinkOut - more resources