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. 2015 Dec;4(4):181-188.

Expression of growth hormone gene during early development of Siberian sturgeon (Acipenserbaerii)

Zeinab Abdolahnejad  1 , Mohammad Pourkazemi  2 , Majid Reza Khoshkholgh  1 , Mahtab Yarmohammadi  3
Affiliations

Expression of growth hormone gene during early development of Siberian sturgeon (Acipenserbaerii)

Zeinab Abdolahnejad et al. Mol Biol Res Commun. 2015 Dec.

Abstract

The mRNA expression of growth hormone (GH) gene in early development stages of Siberian sturgeon was investigated using RT-PCR method. Samples were collected from unfertilized eggs up to 50 days post hatched (dph) larvae in 11 different times. Ribosomal protein L6 (RPL6) transcripts were used as the internal standard during quantification of GH mRNA expression. The results showed that the GH mRNA could be observed in the eyed eggs and even at unfertilized eggs of Siberian sturgeon. The highest amounts of GH mRNA were found at 25 and 50 dph larvae, while the lowest levels were detected at 1 and 3 dph larvae stage. These findings suggest that, the GH mRNA play a key role during developmental stages of Siberian sturgeon.

Keywords: Growth hormone; RT-PCR; Siberian sturgeon; mRNA expression.

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Figures

Figure 1
Figure 1
Relative expression levels of GH mRNA during early development of Siberian sturgeon. Abbreviations: (UF) unfertilized eggs, (E) eyed eggs, (0) newly hatched larvae, (1) 1dph, (2) 3dph, 3-(6dph), 4-(10dph), (5) 15dph, (6) 20dph, (7) 25dph, (8) 50dph.Total RNA was reverse -transcribed and used for quantitative real-time PCR. The relative amount of the Siberian sturgeon GH mRNA was normalized to that level of RPL6 from each RNA sample. Relative GH mRNA expression was analyzed by the 2-ΔΔCT method. The data are shown as mean ± SEM; n=6. Statistical significance of differences of the normalized GH mRNA data between groups was analyzed using one-way ANOVA and followed by Tukey’s test. Different letters indicate significantly different values at P<0.05.
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