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Published Erratum
. 2016 Nov 22;113(47):E7643-E7644.
doi: 10.1073/pnas.1616960113. Epub 2016 Nov 14.

Correction for Ren et al., LRP-6 is a coreceptor for multiple fibrogenic signaling pathways in pericytes and myofibroblasts that are inhibited by DKK-1

No authors listed
Published Erratum

Correction for Ren et al., LRP-6 is a coreceptor for multiple fibrogenic signaling pathways in pericytes and myofibroblasts that are inhibited by DKK-1

No authors listed. Proc Natl Acad Sci U S A. .
No abstract available

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Figures

Fig. 3.
Fig. 3.
DKK-1 blocks pericyte activation and transition to myofibroblasts and reverses myofibroblast activation in vivo, inhibiting fibrogenesis, capillary rarefaction, and inflammation. (A) Western blots of 5 µL of plasma from mice 5 d after i.v. injection of Ad-control or Ad-DKK-1 and from mice subjected to sham surgery and injected with control. (B) Experimental schemata for adenoviral administration, kidney injury, and analysis in the UUO model. (C–M) Prevention studies. (C) Low-magnification confocal images of kidney cortex 4 d after sham operation or UUO in Coll-GFPTr mice that had received Ad-control or Ad-DKK-1 6 d previously, showing Coll-GFP cells or PDGFRβ cells. g, glomerulus; a, arteriole. (D–F) Graphs showing quantification of Coll-GFP cells, PDGFRβ cells, and αSMA cells in kidney 4 d after UUO. (G) Proportion of Coll-GFP cells that express the proliferation marker Ki67. (Hand I) Western blot of GFP (H) or αSMA/CTGF (I) in whole Coll-GFP mouse kidney 4 d after UUO. (J) Quantification of macrophage numbers in kidney sections detected by F4/80 staining. (K) Western blot quantifying canonical WNT signaling by detecting the H2B-GFP fusion protein after Ad-DKK-1 vs. Ad-control treatment of TCF/Lef:H2B-GFPTr reporter mice during UUO kidney injury. (L) Sirius red-stained kidneys 10 d after UUO. (M) Morphometry of Sirius red-stained collagen (Upper) or qPCR for Col1a1transcripts (Lower) 10 d after UUO in mice treated with Ad-control vs. Ad-DKK-1. (N–P) Reversal studies. Confocal Images (N) and morphometric quantification (O) of αSMA staining 10 d after UUO in mice treated with Ad-control or Ad-DKK-1 from day 4. (P) Quantification of capillary density 10 d after UUO. Note that rarefaction occurs in response to kidney disease, but DKK-1 partially reverses rarefaction. (Q) Pericyte detachment. Images and quantification of pericyte area in Coll-GFP mice 2 d after UUO in the presence of circulating DKK-1 or control. Note that injury to the kidney stimulated pericyte spreading and detachment from endothelium (arrowheads). *P < 0.05, **P < 0.01. n = 4–6 per group. Error bars indicate SEM.
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