doi: 10.1016/0022-1759(89)90391-8.
An improved method for the detection of IgE antibody of defined specificity by ELISA using rat monoclonal anti-IgE antibody
Affiliations
- PMID: 2785143
- DOI: 10.1016/0022-1759(89)90391-8
Item in Clipboard
An improved method for the detection of IgE antibody of defined specificity by ELISA using rat monoclonal anti-IgE antibody
J Immunol Methods.
.
Abstract
An improved method for the detection of IgE antibody of defined specificity by ELISA using rat monoclonal anti-IgE antibodies is described. The innovation consists of coating the plates first by a monoclonal rat anti-murine IgE antibody, adding the sera to this antibody-coated plates and then adding the biotin-conjugated antigen after the sera. The plates are then reacted with streptavidin-peroxidase and developed. This procedure eliminates possible competitions with other isotypes of the same specificity. The method is useful especially to quantitate IgE with defined specificity in the presence of high amounts of isotypes of the same specificity.
Similar articles
-
Studies on murine IgE with monoclonal antibodies. I. Characterization of rat monoclonal anti-IgE antibodies and the use of these antibodies for determinations of serum IgE levels and for anaphylactic reactions.Int Arch Allergy Appl Immunol. 1988;85(1):47-54. Int Arch Allergy Appl Immunol. 1988. PMID: 3257463
-
Rapid detection of antigen-specific mouse IgE by enzyme-linked immunosorbent assay (ELISA).J Immunoassay. 1991;12(1):113-24. doi: 10.1080/01971529108055060. J Immunoassay. 1991. PMID: 2040707
-
Measurement of ragweed-specific IgE in canine serum by use of enzyme-linked immunosorbent assays, containing polyclonal and monoclonal antibodies.Am J Vet Res. 1993 Feb;54(2):239-43. Am J Vet Res. 1993. PMID: 8430935
-
Measurement of antigen-specific mouse IgE by a fluorometric reverse (IgE-capture) ELISA.J Immunol Methods. 1989 Jan 17;116(2):181-7. doi: 10.1016/0022-1759(89)90202-0. J Immunol Methods. 1989. PMID: 2783445
-
Evaluation of basic serum IgE levels and the IgE antibody response in the rat by radioimmunoassays.Immunol Rev. 1978;41:261-87. doi: 10.1111/j.1600-065x.1978.tb01468.x. Immunol Rev. 1978. PMID: 360510 Review. No abstract available.
Cited by
-
Construction of a monoclonal antibody against Igh-7b.Immunogenetics. 1994;40(4):296-9. doi: 10.1007/BF00189975. Immunogenetics. 1994. PMID: 8082893 No abstract available.
-
Therapeutic efficacy of spirulina against ovalbumin and cigarette smoke-induced asthma-specific stress biomarkers in Sprague-Dawley rats.Food Sci Nutr. 2022 Nov 14;11(2):972-982. doi: 10.1002/fsn3.3132. eCollection 2023 Feb. Food Sci Nutr. 2022. PMID: 36789047 Free PMC article.
-
Construction of a monoclonal antibody against Igh-7a.Immunogenetics. 1993;37(4):301-4. doi: 10.1007/BF00187459. Immunogenetics. 1993. PMID: 8420839 No abstract available.
-
Transfer of allergic airway responses with antigen-primed CD4+ but not CD8+ T cells in brown Norway rats.J Clin Invest. 1995 Sep;96(3):1303-10. doi: 10.1172/JCI118165. J Clin Invest. 1995. PMID: 7657805 Free PMC article.
-
Human cytomegalovirus (HCMV)-specific immunoglobulin E as a serologic marker for HCMV infection in immunocompromised patients.Clin Investig. 1992 Jun;70(6):497-502. doi: 10.1007/BF00210231. Clin Investig. 1992. PMID: 1327324
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
