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. 2016 Sep;13(5):432-439.
doi: 10.4103/1735-3327.192290.

Socket preservation using freeze-dried bone allograft with and without plasma rich in growth factors in dogs

Mohammad Hasan Samandari  1 , Abbas Haghighat  1 , Nakisa Torabinia  2 , Mehdi Taghian  3 , Leyli Sadri  4 , Vahid Naemy  5
Affiliations

Socket preservation using freeze-dried bone allograft with and without plasma rich in growth factors in dogs

Mohammad Hasan Samandari et al. Dent Res J (Isfahan). 2016 Sep.

Abstract

Background: Plasma rich in growth factors (PRGF) and freeze-dried bone allograft (FDBA) are shown to promote bone healing. This study was aimed to histologically and histomorphometrically investigate the effect of combined use of PRGF and FDBA on bone formation, and compare it to FDBA alone and control group.

Materials and methods: The distal roots of the lower premolars were extracted bilaterally in four female dogs. Sockets were randomly divided into FDBA + PRGF, FDBA, and control groups. Two dogs were sacrificed after 2 weeks and two dogs were sacrificed after 4 weeks. Sockets were assessed histologically and histomorphometrically. Data were analyzed by Kruskal-Wallis test followed by Mann-Whitney U-tests utilizing the SPSS software version 20. P < 0.05 was considered statistically significant.

Results: While the difference in density of fibrous tissue in three groups was not statistically significant (P = 0.343), the bone density in grafted groups was significantly higher than the control group (P = 0.021). The least decrease in all socket dimensions was observed in the FDBA group. However, these differences were only significant in coronal portion at week 4. Regarding socket dimensions and bone density, the difference between FDBA and FDBA+PRGF groups was not significant in middle and apical portions.

Conclusion: The superiority of PRGF+FDBA overFDBA in socket preservation cannot be concluded from this experiment.

Keywords: Allografts; platelet-rich plasma; socket graft.

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Figures

Figure 1
Figure 1
Clinical photograph illustrating distal sockets of mandibular premolars.
Figure 2
Figure 2
Diagram of landmarks used for histomorphometric measurements. LC:Lingual crest of tooth; BC:Buccal crest of tooth; r-r:Rooth length; BB:Thickness of the base of the mandible, a-a:Apical limit of alveolar process; A:Apical limit of periodontal ligament.
Figure 3
Figure 3
Microphotograph of healed sockets. B:Lamellar spongy mature bone(H and E, ×100).
Figure 4
Figure 4
Microphotograph of healed sockets. B:Lamellar spongy mature bone(H and E, ×100).
Figure 5
Figure 5
Mean dimensional changes of three groups at 2 and 4 weeks. Height in mm and cross-sections of coronal, middle, and apical portions in square mm2.
See this image and copyright information in PMC

References

    1. Araújo MG, Lindhe J. Dimensio7nal ridge alterations following tooth extraction. An experimental study in the dog. J Clin Periodontol. 2005;32:212–8. - PubMed
    1. Pietrokovski J, Massler M. Alveolar ridge resorption following tooth extraction. J Prosthet Dent. 1967;17:21–7. - PubMed
    1. Cardaropoli G, Araújo M, Lindhe J. Dynamics of bone tissue formation in tooth extraction sites. An experimental study in dogs. J Clin Periodontol. 2003;30:809–18. - PubMed
    1. Schropp L, Wenzel A, Kostopoulos L, Karring T. Bone healing and soft tissue contour changes following single-tooth extraction: A clinical and radiographic 12-month prospective study. Int J Periodontics Restorative Dent. 2003;23:313–23. - PubMed
    1. Froum S, Cho SC, Rosenberg E, Rohrer M, Tarnow D. Histological comparison of healing extraction sockets implanted with bioactive glass or demineralized freeze-dried bone allograft: A pilot study. J Periodontol. 2002;73:94–102. - PubMed

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