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Editorial
. 2016 Nov 15;594(22):6569-6572.
doi: 10.1113/JP273348.

Are stem cell-derived neural cells physiologically credible?

Andrew D Randall  1
Affiliations
Editorial

Are stem cell-derived neural cells physiologically credible?

Andrew D Randall. J Physiol. .
No abstract available

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Figures

Figure 1
Figure 1. Electrophysiological recordings from iPSC‐derived neurons at physiological temperature
A, example sweeps from a current clamp recording from a typical human iPSC‐derived sensory neuron. Current pulses of 500 ms duration of the indicated amplitudes were applied to cells at rest for the times shown by the arrowheads; note the ability to support robust repetitive firing. B, an example first spike elicited by a depolarizing current injection (left), its first derivative (centre), and a standard phase‐plane plot (right) from a typical iPSC‐derived sensory neuron. Note the fast rising spikes (almost 500 V s−1) and threshold of approximately –40 mV. C, pooled data plotting rate of action potential rise in many iPSC‐derived neurons created from four different control human donors. D, pooled current–voltage relationships for the voltage‐gated Na+ current (open circles) and K+ current (filled squares) recorded from 12 nucleated macropatches isolated from the cell body of iPSC‐derived sensory neurons. E, right, a current clamp recording from an iPSC‐derived forebrain neuron grown for ∼3 weeks in the media described in Telezhkin et al. (2016) within a PuraMatrix‐based 3D culture. Left, a phase plane plot from this neuron for the first action potential fired in response to the depolarizing current injection. Panels A–D, data from A.R. and Malgorzata Mis (University of Bristol); panel E, data from A.R. and Jane Hancock (University of Bristol).
See this image and copyright information in PMC

References

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