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. 2017 Jan 24;61(2):e01542-16.
doi: 10.1128/AAC.01542-16. Print 2017 Feb.

In Vitro Exposure to Increasing Micafungin Concentrations Easily Promotes Echinocandin Resistance in Candida glabrata Isolates

María Ángeles Bordallo-Cardona  1   2 , Pilar Escribano  3   2 , Elia Gómez G de la Pedrosa  4   5 , Laura Judith Marcos-Zambrano  1   2 , Rafael Cantón  4   5 , Emilio Bouza  1   2   6   7 , Jesús Guinea  3   2   6   7
Affiliations

In Vitro Exposure to Increasing Micafungin Concentrations Easily Promotes Echinocandin Resistance in Candida glabrata Isolates

María Ángeles Bordallo-Cardona et al. Antimicrob Agents Chemother. .

Abstract

We assessed the in vitro susceptibility of five echinocandin-susceptible Candida glabrata isolates after exposure to micafungin. The direct exposure to plates at different micafungin concentrations resulted in the inhibition of growth at 0.062 μg/ml. The progressive exposure was performed on plates using 0.031 μg/ml of micafungin and sequential propagation on plates containing the next 2-fold concentration; the MICs of micafungin and anidulafungin increased sequentially, and all the isolates became echinocandin resistant, showing fks2 mutations.

Keywords: Candida glabrata; FKS; echinocandin; fks mutation; micafungin.

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