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. 2016 Nov;23(6):706-712.
doi: 10.1016/j.sjbs.2016.06.010. Epub 2016 Jul 18.

Evaluation of radical scavenging system in two microalgae in response to interactive stresses of UV-B radiation and nitrogen starvation

Sarah A Al-Rashed  1 , Mohamed M Ibrahim  2 , Gehan A El-Gaaly  1 , Sameha Al-Shehri  1 , Ahmed Mostafa  1
Affiliations

Evaluation of radical scavenging system in two microalgae in response to interactive stresses of UV-B radiation and nitrogen starvation

Sarah A Al-Rashed et al. Saudi J Biol Sci. 2016 Nov.

Abstract

The effects of UV-B radiation and/or deprivation of nitrogen stresses on growth rate, some antioxidant compounds, and activities of some antioxidant enzymes, superoxide dismutase (SOD; EC1.15.1.1), ascorbate peroxidase (APx; EC1.11.1.11), guaiacol peroxidase (GUPx; EC1.11.1.7) and glutathione reductase (GR, EC 1.6.4.2), as well as the levels of total glutathione pool, UV-B absorbing pigments, malondialdehyde (MDA) and H2O2 concentrations were studied in Spirulina platensis and Dunaliella salina. Less damage was observed in response to the combined UV-B and nitrogen deprivation as shown by growth rate and photosynthetic pigments especially in Dunaliella salina. A significant increase in flavonoids and phenolics under dual stress was observed. Conversely, a great reduction in malondialdehyde (MDA) and H2O2 concentrations were recorded under the combined stress compared to the effect of each stress. Furthermore, a significant increase in GSH/GSSG ratio toward the control was recorded in response to combined stresses, whereas a significant reduction in this ratio was observed in both microalgae in response to each stress. Increased activities of antioxidant enzymes were recorded under UV-B and nitrogen deprivation stresses.

Keywords: Abiotic stresses; Antioxidant enzymes; Dunaliella salina; Oxidative damage; Spirulina platensis.

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Figures

Image 1
Image 1
Exposure of Spirulina platensis. and Dunaliella salina UV-B and/or N2 starvation treatment (A) before treatment and (B).
See this image and copyright information in PMC

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