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Comment
. 2016 Nov 21;39(4):390-391.
doi: 10.1016/j.devcel.2016.11.005.

Aging Hematopoietic Stem Cells Make Their History

Eva Maria Fast  1 , Leonard Ira Zon  2
Affiliations
Comment

Aging Hematopoietic Stem Cells Make Their History

Eva Maria Fast et al. Dev Cell. .

Abstract

A major hallmark of aging is a decline in tissue regeneration. In a recent issue of Cell, Bernitz and colleagues (2016) determine the divisional history of hematopoietic stem cells (HSCs) to be a key player of regenerative potential in the aging mouse.

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Figures

Figure 1
Figure 1. H2B-GFP Labeling Allows Tracking of Divisional History during Hematopoietic Aging
(A) In the absence of Doxycyclin, cells with active hCD34 promotor will express Histone 2B (H2B) tagged with GFP. Upon addition of Doxycyclin, no new H2B-GFP is being synthesized, leading to dilution of the label upon division. (B) At the onset of the Doxcyclin chase, all phenotypic HSCs (Lineage-Sca1+cKit+CD48-Flk2-CD150+) are GFP+. GFP label gets diluted over time according to divisional activity. Upon transplantation, the label-retaining GFP+ cells contain all the long-term hematopoietic regeneration ability. Adapted from Bernitz et al. (2016).
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