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Multicenter Study
. 2017 Mar-Apr;21(2):185-189.
doi: 10.1016/j.bjid.2016.09.015. Epub 2016 Nov 19.

Complete substitution of the Brazilian endemic clone by other methicillin-resistant Staphylococcus aureus lineages in two public hospitals in Rio de Janeiro, Brazil

Raiane Cardoso Chamon  1 , Sthefanie da Silva Ribeiro  1 , Thaina Miranda da Costa  1 , Simone Aranha Nouér  2 , Katia Regina Netto Dos Santos  3
Affiliations
Multicenter Study

Complete substitution of the Brazilian endemic clone by other methicillin-resistant Staphylococcus aureus lineages in two public hospitals in Rio de Janeiro, Brazil

Raiane Cardoso Chamon et al. Braz J Infect Dis. 2017 Mar-Apr.

Abstract

Staphylococcus aureus is an important cause of bloodstream infections. Therefore, the main purpose of this work was to characterize a collection of 139 S. aureus isolates from bloodstream infections in two public hospitals in relation to their antimicrobial susceptibility profile, staphylococcal cassette chromosome mec types, and clonal relationship. Methicillin resistance and resistance to other 12 agents were accessed by the disk diffusion test. Minimum inhibitory concentration to mupirocin was also determined. The SCCmec types were accessed by multiplex PCR, and the clonal relationship was determined by pulsed field gel electrophoresis method and restriction modification system characterization. Besides, multilocus sequence typing was performed for representative methicillin-resistant S. aureus isolates. The military hospital showed a dissemination of the New York/Japan (USA100/ST5/CC5/SCCmecII) lineage associated to multidrug resistance, including mupirocin resistance, and the teaching hospital presented polyclonal and non-multidrug resistant MRSA isolates. Complete substitution of the Brazilian endemic clone by other lineages was found in both hospitals. These findings can highlight differences in policy control and prevention of infections used in the hospitals and a change in the epidemiological profile of MRSA in Brazilian hospitals, with the replacement of BEC, a previously well-established clone, by other lineages.

Keywords: Bloodstream infections; Mupirocin resistance; Staphylococcus aureus; USA100.

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References

    1. Rhee Y., Aroutcheva A., Hota B., et al. Evolving epidemiology of Staphylococcus aureus bacteremia. Infect Control Hosp Epidemiol. 2015;36:1417–1422. - PubMed
    1. Liu Q., Han L., Li B., et al. Virulence characteristic and MLST-agr genetic background of high-level mupirocin-resistant, MRSA isolates from Shanghai and Wenzhou, China. PLoS ONE. 2012;7:e37005. - PMC - PubMed
    1. Caboclo R.M., Cavalcante F.S., Iorio N.L., et al. Methicillin-resistant Staphylococcus aureus in Rio de Janeiro hospitals: dissemination of the USA400/ST1 and USA800/ST5 SCCmec type IV and USA100/ST5 SCCmec type II lineages in a public institution and polyclonal presence in a private one. Am J Infect Control. 2013;41:e21–e26. - PubMed
    1. Schuenck R.P., Cavalcante F.S., Emery E., et al. Staphylococcus aureus isolates belonging to different multilocus sequence types present specific virulence gene profiles. FEMS Immunol Med Microbiol. 2012;65:501–504. - PubMed
    1. Caiaffa-Filho H.H., Trindade P.A., da Cunha G.P., et al. Methicillin-resistant Staphylococcus aureus carrying SCCmec type II was more frequent than the Brazilian endemic clone as a cause of nosocomial bacteremia. Diagn Microbiol Infect Dis. 2013;76:518–520. - PubMed

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