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. 2016 Oct 19;13(11):853-857.
doi: 10.7150/ijms.16706. eCollection 2016.

Amelioration of estrogen deficiency-induced obesity by collagen hydrolysate

Tsay-I Chiang  1 , I-Chang Chang  2 , Hsueh-Hui Lee  3 , Kuang Hui Hsieh  3 , Yung-Wei Chiu  4 , Te-Jen Lai  5 , Jer-Yuh Liu  6 , Li-Sung Hsu  7 , Shao-Hsuan Kao  7
Affiliations

Amelioration of estrogen deficiency-induced obesity by collagen hydrolysate

Tsay-I Chiang et al. Int J Med Sci. .

Abstract

Objectives: Menopausal transition with declining estrogen levels significantly affects the physiological properties of women and consequently contributes to a series of medical conditions, including obesity. Obesity is a crucial risk factor associated with cardiovascular diseases, diabetes mellitus, and breast cancer. Increasing dietary protein content improves satiety and energy expenditure. Thus, we hypothesize that supplementing with collagen, a common dietary protein, may alleviate menopause-induced obesity. Methods: We used ovariectomized (OVX) rats to mimic a menopausal human. The body weight of OVX rats significantly increased compared with that of sham-operated rats (P<0.05), but uterus weight was decreased. Adipocyte size in perigonadal adipose tissue also increased (P<0.05). Results: By contrast, OVX rats supplemented with aqueous collagen hydrolysate (2.5 mg/mL) exhibited significant attenuation in body weight gain and adipocyte enlargement (P<0.05), but insignificant change in uterus weight. Further investigation indicated that collagen hydrolysate supplementation insignificantly affected the levels of dorsal fat, serum total cholesterol, and serum triacylglycerol. Levels of serum biochemical factors, calcium, phosphorus, and glucose were also insignificantly altered by collagen hydrolysate supplementation. Conclusion: Collagen hydrolysate supplementation reduced body weight gain and adipocyte enlargement in response to ovariectomy but slightly affected blood lipids, calcium, and glucose in both sham-operated and OVX rats. Collagen hydrolysate supplementation is beneficial in ameliorating estrogen deficiency-induced obesity and its associated risk factors.

Keywords: Collagen; Obesity; estrogen deficiency.

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Conflict of interest statement

The authors have declared that no competing interest exists.

Figures

Figure 1
Figure 1
Effects of collagen supplement on body weights. Rats were sham-operated or OVX, and then the OVX-rats were supplied with water containing collagen (1.25 and 2.5 mg/mL, 1.25C and 2.5C) or not. (A) Body weight was measured every week for 12 weeks. (B) After 12 weeks, rats were sacrificed, and then uteruses were obtained and weighed. #, P<0.05 as compared to Sham. *, P<0.05 as compared to OVX.
Figure 2
Figure 2
Effects of collagen supplement on adipocyte size and dorsal fat content. Rats were sham-operated or OVX, and then the OVX-rats were supplied with water containing collagen (1.25 and 2.5 mg/mL, 1.25C and 2.5C) or not. After 12 weeks, the rats were weighed, and then sacrificed to obtain periuterine adipose tissue and dorsal fat. (A) After H&E staining, the adipocytes were observed by using light microscope (200x). (B) Dorsal fat samples were collected and weighed.
Figure 3
Figure 3
Collagen supplement insignificantly affected blood lipids. Rats were sham-operated or OVX, and then the OVX-rats were supplied with water containing collagen (1.25 and 2.5 mg/mL, 1.25C and 2.5C) or not. After 12 weeks, blood samples were obtained for determination of serum total cholesterol and triacylglycerol. n.s., no statistical significance as compared to Sham or OVX.
Figure 4
Figure 4
Collagen supplement insignificantly affected blood lipids. Rats were sham-operated or OVX, and then the OVX-rats were supplied with water containing collagen (1.25 and 2.5 mg/mL, 1.25C and 2.5C) or not. After 12 weeks, sera were obtained for determination of calcium, phosphorus and glucose level. n.s., no statistical significance as compared to Sham or OVX.
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