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. 2015 Aug 13;16(4):045005.
doi: 10.1088/1468-6996/16/4/045005. eCollection 2015 Aug.

Impact of monoolein on aquaporin1-based supported lipid bilayer membranes

Zhining Wang  1 , Xida Wang  1 , Wande Ding  1 , Miaoqi Wang  1 , Xin Qi  2 , Congjie Gao  1
Affiliations

Impact of monoolein on aquaporin1-based supported lipid bilayer membranes

Zhining Wang et al. Sci Technol Adv Mater. .

Abstract

Aquaporin (AQP) based biomimetic membranes have attracted considerable attention for their potential water purification applications. In this paper, AQP1 incorporated biomimetic membranes were prepared and characterized. The morphology and structure of the biomimetic membranes were characterized by in situ atomic force microscopy (AFM), infrared absorption spectroscopy, fluorescence microscopy, and contact angle measurements. The nanofiltration performance of the AQP1 incorporated membranes was investigated at 4 bar by using 2 g l-1 NaCl as feed solution. Lipid mobility plays an important role in the performance of the AQP1 incorporated supported lipid bilayer (SLB) membranes. We demonstrated that the lipid mobility is successfully tuned by the addition of monoolein (MO). Through in situ AFM and fluorescence recovery after photo-bleaching (FRAP) measurements, the membrane morphology and the molecular mobility were studied. The lipid mobility increased in the sequence DPPC < DPPC/MO (RMO = 5/5) < DOPC/MO (RMO = 5/5) < DOPC, which is consistent with the flux increment and salt rejection. This study may provide some useful insights for improving the water purification performance of biomimetic membranes.

Keywords: aquaporin; biomimetic membrane; lipid mobility; monoolein; water purification.

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Figures

Figure 1.
Figure 1.
In situ AFM images (scan size 5 × 5 μm2, z-scale 500 nm) of (A) NTR7450 and (B) NF270.
Figure 2.
Figure 2.
In situ AFM images (scan size 5 × 5 μm2, z-scale 500 nm) of DPPC/MO vesicles deposited on NTR7450 surface with different RMO values, (A) RMO = 0, (B) RMO = 3/7, (C) RMO = 5/5 and (D) RMO = 7/3; DOPC/MO vesicles deposited on NTR7450 surface with different RMO, (E) RMO = 0, (F) RMO = 3/7, (G) RMO = 5/5 and (H) RMO = 7/3.
Figure 3.
Figure 3.
In situ AFM images (scan size 5 × 5 μm2, z-scale 500 nm) of DPPC/MO vesicles deposited on NF270 surface with different RMO values, (A) RMO = 0, (B) RMO = 3/7, (C) RMO = 5/5 and (D) RMO = 7/3; DOPC/MO vesicles deposited on NF270 surface with different RMO, (E) RMO = 0, (F) RMO = 3/7, (G) RMO = 5/5 and (H) RMO = 7/3.
Figure 4.
Figure 4.
FTIR spectra of NF membrane supported lipid membranes. Top: NTR7450 SLBs (RMO = 5/5) membrane (A); bottom: NF270 SLBs (RMO = 5/5) membrane (B).
Figure 5.
Figure 5.
Confocal fluorescence images of NTR7450 supported DPPC/MO and DOPC/MO bilayers with 0.5 wt% NBD-PE. (A) DPPC (RMO = 0), (B) DPPC/MO (RMO = 5/5), (C) DOPC (RMO = 0), (D) DOPC/MO (RMO = 5/5). Inset of (A) is the fluorescence image of virgin NTR7450.
Figure 6.
Figure 6.
Confocal fluorescence images of NF270 supported DPPC/MO and DOPC/MO bilayers with 0.5 wt% NBD-PE. (A) DPPC (RMO = 0), (B) DPPC/MO (RMO = 5/5), (C) DOPC (RMO = 0), (D) DOPC/MO (RMO = 5/5). Inset of (A) is the fluorescence image of virgin NF270.
Figure 7.
Figure 7.
The FRAP curves of NTR7450 (A) and NF270 (B) SLB membranes.
Figure 8.
Figure 8.
Contact angles of NTR7450 and NF270 SLB membranes. Each point represents the mean ± standard deviation of three samples.
Figure 9.
Figure 9.
Effect of different content of MO SLBs on the relative pure water flux of SLB membranes.
Figure 10.
Figure 10.
Pure water flux of NTR7450 and NF270 supported DPPC, DPPC/MO (RMO = 5/5), DOPC, and DOPC/MO (RMO = 5/5) bilayer membranes with different RAQP1.
Figure 11.
Figure 11.
Water flux (A) and NaCl rejection (B) of NTR7450 supported DPPC, DPPC/MO (RMO = 5/5), DOPC, and DOPC/MO (RMO = 5/5) bilayer membranes with different RAQP1.
Figure 12.
Figure 12.
Water flux (A) and NaCl rejection (B) of NF270 supported DPPC, DPPC/MO (RMO = 5/5), DOPC, and DOPC/MO (RMO = 5/5) bilayer membranes with different RAQP1.
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