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. 2016 Nov;12(11):20160628.
doi: 10.1098/rsbl.2016.0628.

Zebrafish embryonic development is induced by carp sperm

Thomas A Delomas  1 , Konrad Dabrowski  2
Affiliations

Zebrafish embryonic development is induced by carp sperm

Thomas A Delomas et al. Biol Lett. 2016 Nov.

Abstract

Haploid gynogenetic screens increase the efficiency of forward genetic screens and linkage analysis in fish. Typically, UV-irradiated zebrafish sperm is used to activate zebrafish oocytes for haploid screens. We describe the use of UV-irradiated common carp sperm to activate haploid gynogenetic zebrafish development. Carp × zebrafish hybrids are shown to have a characteristic set of features during embryonic development and exhibit functional development of several tissues (muscle, heart and nervous system). Hybrids become inviable past the embryonic stages. This technique eliminates the possibility of incompletely irradiated zebrafish spermatozoa contaminating haploid progenies. While developing this protocol, one unique zebrafish female was identified which, upon insemination with UV-irradiated carp spermatozoa, repeatedly displayed spontaneous diploidization of the maternal chromosomes in her offspring.

Keywords: forward genetic screen; gynogenesis; haploid screen; spontaneous diploid.

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Figures

Figure 1.
Figure 1.
Average per cent of embryos surviving in propagations of wild-type females (not high frequency spontaneous diploidization of maternal chromosomes, SDM) from the first mitotic division (2-cell). Error bars represent standard deviation and only one direction is shown to avoid displaying overlapping bars.
Figure 2.
Figure 2.
Development of (a) haploid zebrafish, (b) zebrafish × carp hybrids and (c) diploid zebrafish.
See this image and copyright information in PMC

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