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. 1989 Jul 26;121(2):281-8.
doi: 10.1016/0022-1759(89)90172-5.

A fluorescence sandwich ELISA for detecting soluble and cell-associated human interleukin-2

K Kitamura  1 , K Matsuda, M Ide, T Tokunaga, M Honda
Affiliations

A fluorescence sandwich ELISA for detecting soluble and cell-associated human interleukin-2

K Kitamura et al. J Immunol Methods. .

Abstract

A fluorescence sandwich ELISA was developed for the quantitation of human interleukin-2 (IL-2) using a combination of two anti-human IL-2 antibodies, L61 and biotinylated anti-IL-2 rabbit IgG. The minimum level of IL-2 detectable by this ELISA was 0.3 pg/ml, indicating that this method is about 100 times more sensitive than the usual colorimetric human IL-2 ELISA. The ELISA specifically detected human IL-2 in cell culture supernatants and serum, and also in extracts from mitogen-stimulated peripheral blood mononuclear cells. All of the IL-2 species detected in the supernatants, sera and cell lysates were shown to have a molecular weight of 15,000, as estimated by size exclusion high performance liquid chromatography.

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