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. 2016 Nov;12(5):2865-2868.
doi: 10.3892/etm.2016.3692. Epub 2016 Sep 9.

Increased apoptosis rate of human decidual cells and cytotrophoblasts in patients with recurrent spontaneous abortion as a result of abnormal expression of CDKN1A and Bax

Xiaomei Lv  1 , Zhenhong Cai  1 , Su Li  1
Affiliations

Increased apoptosis rate of human decidual cells and cytotrophoblasts in patients with recurrent spontaneous abortion as a result of abnormal expression of CDKN1A and Bax

Xiaomei Lv et al. Exp Ther Med. 2016 Nov.

Abstract

In the present study, we analyzed the proliferation and apoptosis of trophoblasts and human decidual cells in patients with recurrent spontaneous abortion and the related cellular pathway mechanism. Thirty-four patients with recurrent abortion and 30 healthy pregnant women undergoing planned artificial abortion were selected. The trophoblast and decidual cells were collected by negative pressure aspiration technique. TUNEL method was used to detect the apoptosis rate. Immunohistochemical method was used for detection of TP53 protein. Quantitative real-time PCR was used for detection of the relative expression level of CDKN1A and Bax mRNA. It was found that the cell apoptosis rate in the recurrent miscarriage group was significantly increased and the expression levels of TP53 protein, CDKN1A and Bax mRNA were also significantly increased (p<0.05). In conclusion, the trophoblast and decidual cells of patients with recurrent abortion were obviously apoptotic, which was probably related to abnormal expression of the CDKN1A and Bax genes mediated by TP53 protein through cellular pathways.

Keywords: BAX; CDKN1A; TP53 protein; apoptosis; recurrent abortion.

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Figures

Figure 1.
Figure 1.
Apoptosis rate as detected by TUNEL method (magnification, 100x). (A) Control group and (B) recurrent abortion group.
Figure 2.
Figure 2.
Immunohistochemical staining for TP53 protein (magnification, 200x). (A) Control group and (B) recurrent abortion group.
See this image and copyright information in PMC

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