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. 2016 Nov 28:6:37870.
doi: 10.1038/srep37870.

Identification and expression analysis of an olfactory receptor gene family in green plant bug Apolygus lucorum (Meyer-Dür)

Xing-Kui An  1 , Liang Sun  1   2 , Hang-Wei Liu  1 , Dan-Feng Liu  1 , Yu-Xiao Ding  1   3 , Le-Mei Li  1 , Yong-Jun Zhang  1 , Yu-Yuan Guo  1
Affiliations

Identification and expression analysis of an olfactory receptor gene family in green plant bug Apolygus lucorum (Meyer-Dür)

Xing-Kui An et al. Sci Rep. .

Abstract

Olfactory receptors are believed to play a central role in insects host-seeking, mating, and ovipositing. On the basis of male and female antennal transcriptome of adult Apolygus lucorum, a total of 110 candidate A. lucorum odorant receptors (AlucOR) were identified in this study including five previously annotated AlucORs. All the sequences were validated by cloning and sequencing. Tissue expression profiles analysis by RT-PCR indicated most AlucORs were antennal highly expressed genes. The qPCR measurements further revealed 40 AlucORs were significantly higher in the antennae. One AlucOR was primarily expressed in the female antennae, while nine AlucORs exhibited male-biased expression patterns. Additionally, both the RPKM value and RT-qPCR analysis showed AlucOR83 and AlucOR21 were much higher abundant in male antennae than in female antennae, suggesting their different roles in chemoreception of gender. Phylogenetic analysis of ORs from several Hemipteran species demonstrated that most AlucORs had orthologous genes, and five AlucOR-specific clades were defined. In addition, a sub-clade of potential male-based sex pheromone receptors were also identified in the phylogenetic tree of AlucORs. Our results will facilitate the functional studies of AlucORs, and thereby provide a foundation for novel pest management approaches based on these genes.

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Figures

Figure 1
Figure 1. The length distribution of the assembled unigenes from A. lucorum male and female antennal transcriptome.
Figure 2
Figure 2. Species distribution of unigenes’ best-hit annotation term in nr database.
Figure 3
Figure 3. Gene ontology classifications of the A. lucorum unigenes.
Figure 4
Figure 4. Neighbor-joining tree of candidate OR proteins (>200 aa) from Hemiptera species.
The evolutionary distances were computed using the p-distance method. Afas: Adelphocoris fasciaticollis; Alin: Adelphocoris lineolatus; Asut: Adelphocoris suturalis; Aluc: Apolygus lucorum; Hhal: Halyomorpha halys; Clec: Cimex lectularius; Sfur: Sogatella furcifera.
Figure 5
Figure 5. Tissues expression profiles of candidate AlucORs evaluated by RT-PCR.
FA: female antennae; MA: male antennae; H: heads; B: body parts without heads including body parts including thoraxes, abdomens, legs, and wings.
See this image and copyright information in PMC

References

    1. Hansson Bill S. & Stensmyr Marcus C. Evolution of Insect Olfaction. Neuron 72, 698–711 (2011). - PubMed
    1. Leal W. S. Odorant reception in insects: roles of receptors, binding proteins, and degrading enzymes. Annu. Rev. Entomol. 58, 373–391 (2013). - PubMed
    1. Touhara K. & Vosshall L. B. Sensing odorants and pheromones with chemosensory receptors. Annu. Rev. Physiol. 71, 307–332 (2009). - PubMed
    1. Zhao X. C. et al.. Fine structure and primary sensory projections of sensilla located in the labial-palp pit organ of Helicoverpa armigera (Insecta). Cell Tissue Res 353, 399–408 (2013). - PubMed
    1. Gao Q. & Chess A. Identification of candidate Drosophila olfactory receptors from genomic DNA sequence. Genomics 60, 31–39 (1999). - PubMed

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