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Review
. 2017:1546:57-73.
doi: 10.1007/978-1-4939-6730-8_4.

LC-MSE, Multiplex MS/MS, Ion Mobility, and Label-Free Quantitation in Clinical Proteomics

Gustavo Henrique Martins Ferreira Souza  1 , Paul C Guest  2 , Daniel Martins-de-Souza  2
Affiliations
Review

LC-MSE, Multiplex MS/MS, Ion Mobility, and Label-Free Quantitation in Clinical Proteomics

Gustavo Henrique Martins Ferreira Souza et al. Methods Mol Biol. 2017.

Abstract

Proteomic tools can only be implemented in clinical settings if high-throughput, automated, sensitive, and accurate methods are developed. This has driven researchers to the edge of mass spectrometry (MS)-based proteomics capacity. Here we provide an overview of recent achievements in mass spectrometric technologies and instruments. This includes development of high and ultra definition-MSE (HDMSE and UDMSE) through implementation of ion mobility (IM) MS towards sensitive and accurate label-free proteomics using ultra performance liquid chromatography (UPLC). Label free UPLC-HDMSE is less expensive than labeled-based quantitative proteomics and has no limits regarding the number of samples that can be analyzed and compared, which is an important requirement for supporting clinical applications.

Keywords: DIA; Data independent acquisition; HDMSE; Label-free; MSE; Proteomics; UDMSE.

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