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. 2016;5(2):S0052.
doi: 10.5702/massspectrometry.S0052. Epub 2016 Nov 25.

Technical Challenges in Mass Spectrometry-Based Metabolomics

Fumio Matsuda  1
Affiliations

Technical Challenges in Mass Spectrometry-Based Metabolomics

Fumio Matsuda. Mass Spectrom (Tokyo). 2016.

Abstract

Metabolomics is a strategy for analysis, and quantification of the complete collection of metabolites present in biological samples. Metabolomics is an emerging area of scientific research because there are many application areas including clinical, agricultural, and medical researches for the biomarker discovery and the metabolic system analysis by employing widely targeted analysis of a few hundred preselected metabolites from 10-100 biological samples. Further improvement in technologies of mass spectrometry in terms of experimental design for larger scale analysis, computational methods for tandem mass spectrometry-based elucidation of metabolites, and specific instrumentation for advanced bioanalysis will enable more comprehensive metabolome analysis for exploring the hidden secrets of metabolism.

Keywords: absolute quantification; experimental design; in silico fragmentation; metabolomics; small molecule identification.

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References

    1. 1) G. A. Theodoridis, H. G. Gika, E. J. Want, I. D. Wilson. Liquid chromatography-mass spectrometry based global metabolite profiling: A review. Anal. Chim. Acta 711: 7–16, 2012. - PubMed
    1. 2) C. H. Johnson, J. Ivanisevic, G. Siuzdak. Metabolomics: Beyond biomarkers and towards mechanisms. Nat. Rev. Mol. Cell Biol. 17: 451–459, 2016. - PMC - PubMed
    1. 3) G. A. Gowda, S. Zhang, H. Gu, V. Asiago, N. Shanaiah, D. Raftery. Metabolomics-based methods for early disease diagnostics. Expert Rev. Mol. Diagn. 8: 617–633, 2008. - PMC - PubMed
    1. 4) M. A. Kamleh, K. Spagou, E. J. Want. Metabolic profiling in disease diagnosis, toxicology and personalized healthcare. Curr. Pharm. Biotechnol. 12: 976–995, 2011. - PubMed
    1. 5) S. Harada, T. Takebayashi, A. Kurihara, M. Akiyama, A. Suzuki, Y. Hatakeyama, D. Sugiyama, K. Kuwabara, A. Takeuchi, T. Okamura, Y. Nishiwaki, T. Tanaka, A. Hirayama, M. Sugimoto, T. Soga, M. Tomita. Metabolomic profiling reveals novel biomarkers of alcohol intake and alcohol-induced liver injury in community-dwelling men. Environ. Health Prev. Med. 21: 18–26, 2016. - PMC - PubMed