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. 2016 Dec;42(4):285-289.
doi: 10.5152/tud.2016.98474.

Distribution of interstitial cells of Cajal in the bladders of fetal rats with retinoic acid induced myelomeningocele

Ali Tekin  1 , Osman Zeki Karakuş  2 , Gülce Hakgüder  2 , Oğuz Ateş  2 , Erdener Özer  3 , Mustafa Olguner  2 , Feza Miraç Akgür  2
Affiliations

Distribution of interstitial cells of Cajal in the bladders of fetal rats with retinoic acid induced myelomeningocele

Ali Tekin et al. Turk J Urol. 2016 Dec.

Abstract

Objective: Myelomeningocele (MMC) is one of the most common reason of neurogenic bladder dysfunction in children. Although neurogenic bladder dysfunction occurrence is related with bladder innervation, also there are some changes seen in the smooth muscle and neural cells of the bladder. Interstitial cells of Cajal (ICC) are the pacemaker cells found in organs with peristaltic activity. Although it has been shown that ICC are diminished in the rat urinary bladder with traumatic spinal cord injury, there is no data about ICC in fetal rat bladders with MMC. This study has been conducted to investigate the ICC in the bladders of fetal rats with retinoic acid induced MMC.

Materials and methods: Time dated pregnant Wistar albino rats were divided into 3 groups. In MMC group, dams were fed with gavage solution containing 60 mg/kg all-trans retinoic acid dissolved in olive oil on 10. embryologic day. Sham group animals were fed only olive oil. Control group dams were fed with standard rat chow. Fetuses were delivered by cesarean section and harvested on 22. embryologic day. MMC was identified by observing MMC sacs at the back of the fetuses. Distribution of ICCs were evaluated using immunohistochemical staining.

Results: ICCs were found in all groups, which have the same morphological features that had been described earlier in the gastrointestinal tract and the bladder. The density of the ICC in the MMC group was found to be significantly decreased when compared with the control and the sham groups (p<0.05).

Conclusion: The density of the ICC in the urinary bladder decreased in the neurogenic bladder developed in MMC.

Keywords: Bladder contractility; bladder dysfunction; interstitial cells of Cajal; myelomeningocele; neurogenic bladder.

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Conflict of interest statement

No conflict of interest was declared by the authors.

Figures

Figure 1
Figure 1
Macroscopic appearance of rat fetus with myelomeningocele
Figure 2
Figure 2
Histopathology of a fetal rat bladder with an arrow indicating a mast cell (C-kit, ×40)
Figure 3
Figure 3
Histopathology of the fetal rat bladder of the control group (arrow indicates interstitial cells of Cajal located in the muscular layer with fusiform cell body, thin cytoplasm, wide ovoid nucleus and two dentritic processes) (C-kit, ×40)
Figure 4
Figure 4
Histopathology of the fetal rat bladder of the myelomeningocele group (arrow indicates mast cells) (Toluidin blue, ×40)
Figure 5
Figure 5
Scores of interstitial cells of Cajal in all groups (*myelomeningocele) *p<0.05. When compared with the control and sham groups.
See this image and copyright information in PMC

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