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. 2016 Dec 1;21(12):1652.
doi: 10.3390/molecules21121652.

Multi-Residue Analysis of Pesticide Residues in Crude Pollens by UPLC-MS/MS

Zhou Tong  1 , Yan-Can Wu  2   3 , Qiong-Qiong Liu  4 , Yan-Hong Shi  5 , Li-Jun Zhou  6 , Zhen-Yu Liu  7 , Lin-Sheng Yu  8 , Hai-Qun Cao  9
Affiliations

Multi-Residue Analysis of Pesticide Residues in Crude Pollens by UPLC-MS/MS

Zhou Tong et al. Molecules. .

Abstract

A multi-residue method for the determination of 54 pesticide residues in pollens has been developed and validated. The proposed method was applied to the analysis of 48 crude pollen samples collected from eight provinces of China. The recovery of analytes ranged from 60% to 136% with relative standard deviations (RSDs) below 30%. Of the 54 targeted compounds, 19 pesticides were detected. The major detection rates of each compound were 77.1% for carbendazim, 58.3% for fenpropathrin, 56.3% for chlorpyrifos, 50.0% for fluvalinate, 31.3% for chlorbenzuron, and 29.2% for triadimefon in crude pollen samples. The maximum values of each pesticide were 4516 ng/g for carbendazim, 162.8 ng/g for fenpropathrin, 176.6 ng/g for chlorpyrifos, 316.2 ng/g for fluvalinate, 437.2 ng/g for chlorbenzuron, 79.00 ng/g for triadimefon, and so on. This study provides basis for the research on the risks to honeybee health.

Keywords: UPLC-MS/MS; multi-residue; pesticide; pollen.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
An example of the extracted Quantification ion (MRM1) chromatograms of a pollen sample, indicating the presence of (A) carbendazim; (B) fenpropathrin; (C) chlorpyrifos; and (D) triadimefon.
Figure 2
Figure 2
Location of the region of the sample collected in China: A—Jilin Province; B—Shanxi Province; C—Shandong Province; D—Henan Province; E—Anhui Province; F—Hubei Province; G—Chongqing Province; H—Hainan Province.
See this image and copyright information in PMC

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