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. 2017 Jan 24;61(2):e01856-16.
doi: 10.1128/AAC.01856-16. Print 2017 Feb.

Genetic Environment of the blaKPC-2 Gene in a Klebsiella pneumoniae Isolate That May Have Been Imported to Russia from Southeast Asia

Vladimir Ageevets  1 , Julia Sopova  2   3 , Irina Lazareva  1 , Maya Malakhova  4 , Elena Ilina  4 , Elena Kostryukova  4 , Vladislav Babenko  4 , Alessandra Carattoli  5 , Yuri Lobzin  1   6 , Alexander Uskov  1 , Sergey Sidorenko  7   6
Affiliations

Genetic Environment of the blaKPC-2 Gene in a Klebsiella pneumoniae Isolate That May Have Been Imported to Russia from Southeast Asia

Vladimir Ageevets et al. Antimicrob Agents Chemother. .

Abstract

The nucleotide sequence of a blaKPC-2-harboring plasmid (pKPCAPSS) from Klebsiella pneumoniae ST273 isolated in Saint Petersburg, Russia, from a patient with history of recent travel to Vietnam is presented. This 127,970-bp plasmid possessed both IncFII and IncR replicons. blaKPC-2 was localized on a hypothetical mobile element. This element was flanked by 38-bp inverted Tn3 repeats and included a Tn3-specific transposase gene, macrolide resistance operon (mphA-mrx-mphR), and a fragment of blaTEM with unique polymorphisms.

Keywords: IncFII; IncR; KPC-2; Klebsiella pneumoniae; transposons.

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Figures

FIG 1
FIG 1
Genetic map and comparison of the hypothetical mobile element from plasmid pKPCAPSS with the blaKPC-2 gene environment from plasmids, pKPC3_SZ (11) and pKP048 (9) and with the Tn3 transposon (23). The hypothetical mobile element was flanked by inverted Tn3 repeats with the following gene order: Tn3-specific transposase gene (green), macrolide resistance operon genes mphA, mrx, and mphR (yellow), tnpR and tnpA genes identical to those of pKPC3_SZ and pKP048 (blue), insertion of the ΔTEM-1 fragment with SNPs (green), noncoding region (NCR211), fragment identical to Tn4401 that contained the blaKPC-2 gene and ISKpn6 (red), genes encoding antirestriction proteins (gray), and a gene encoding a replication protein (blue). The part of the Tn3 transposon containing the tnpR and blaTEM-1 genes flanked this construction (green).
See this image and copyright information in PMC

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