Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017:1542:321-333.
doi: 10.1007/978-1-4939-6707-0_21.

PCR ITS-RFLP for Penicillium Species and Other Genera

Affiliations

PCR ITS-RFLP for Penicillium Species and Other Genera

Sandrine Rousseaux et al. Methods Mol Biol. 2017.

Abstract

Among numerous molecular methodologies developed for highly specific identification of filamentous fungi isolates, here we describe restriction digestion analysis of the ITS products as an easy method to identify isolates of filamentous fungi. This technique is a rapid and reliable method appropriate for routine identification of filamentous fungi. This can be used to screen large numbers of isolates from various environments in a short time. The use of different endonucleases allowed generating individual restriction profiles. The individual profiles obtained were combined into composite restriction patterns characteristic of a species. Eleven different genera can be differentiated and among them 41 different species.

Keywords: Composite profiles; Filamentous fungi; Identification; PCR ITS-RFLP.

PubMed Disclaimer

MeSH terms

Substances

LinkOut - more resources