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. 2017 Mar:48:19-26.
doi: 10.1016/j.meegid.2016.12.002. Epub 2016 Dec 6.

Candidate new rotavirus species in Schreiber's bats, Serbia

Krisztián Bányai  1 , Gábor Kemenesi  2 , Ivana Budinski  3 , Fanni Földes  2 , Brigitta Zana  2 , Szilvia Marton  4 , Renáta Varga-Kugler  4 , Miklós Oldal  2 , Kornélia Kurucz  2 , Ferenc Jakab  5
Affiliations

Candidate new rotavirus species in Schreiber's bats, Serbia

Krisztián Bányai et al. Infect Genet Evol. 2017 Mar.

Abstract

The genus Rotavirus comprises eight species designated A to H and one tentative species, Rotavirus I. In a virus metagenomic analysis of Schreiber's bats sampled in Serbia in 2014 we obtained sequences likely representing novel rotavirus species. Whole genome sequencing and phylogenetic analysis classified the representative strain into a tentative tenth rotavirus species, we provisionally called Rotavirus J. The novel virus shared a maximum of 50% amino acid sequence identity within the VP6 gene to currently known members of the genus. This study extends our understanding of the genetic diversity of rotaviruses in bats.

Keywords: Astrovirus; Chiroptera; Coronavirus; Gemycircularvirus; Miniopterus schreibersii; Retrovirus; Rotavirus; Semiconductor sequencing; Viral metagenomics.

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Figures

Fig. 1.
Fig. 1
Distribution of viral sequence reads in six bat fecal specimens.
Fig. 2.
Fig. 2
Phylogenetic trees obtained for the genes encoding all major structural proteins (VP1 to VP4, VP6, and VP7) and non-structural proteins (NSP1 to NSP5) with representative strains of RVA to RVI. Alignments were created using the TranslatorX online platform (http://translatorx.co.uk/). Phylogenetic trees were prepared using the maximum likelihood method as implemented in Mega6 (http://www.megasoftware.net/). Bootstrap values are shown at the branch nodes. Calibration bars are proportional to the genetic distance.
Fig. 3.
Fig. 3
Phylogenetic analysis of the VP6 gene. A total of 258 representative amino acid sequences were selected to provide a more comprehensive phylogenetic analysis for the VP6 gene. Color codes are indicated below the tree. Bootstrap values at the deeper nodes are shown. Calibration bar is proportional to the genetic distance.
Fig. 4.
Fig. 4
Similarity plot prepared from amino acid sequences of the VP6 protein. Dashed line indicates the rotavirus species demarcation sequence identity cut-off value determined by Matthijnssens et al. (2012). Color codes are indicated below the plot.
See this image and copyright information in PMC

References

    1. Abascal F., Zardoya R., Telford M.J. TranslatorX: multiple alignment of nucleotide sequences guided by amino acid translations. Nucleic Acids Res. 2010;38:W7–13. - PMC - PubMed
    1. Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. Basic local alignment search tool. J. Mol. Biol. 1990;215:403–410. - PubMed
    1. Appleton B.R., McKenzie J.A., Christidis L. Molecularsystematics and biogeography of the bent-wing bat complex Miniopterus schreibersii (Kuhl, 1817) (Chiroptera: Vespertilionidae) Mol. Phylogenet. Evol. 2004;31:431–439. - PubMed
    1. Asano K.M., Gregori F., Hora A.S., Scheffer K.C., Fahl W.O., Iamamoto K., Mori E., Silva F.D., Taniwaki S.A., Brandão P.E. Group A rotavirus in Brazilian bats: description of novel T15 and H15 genotypes. Arch. Virol. 2016;161:3225–3230. - PubMed
    1. Buchfink B., Xie C., Huson D.H. Fast and sensitive protein alignment using DIAMOND. Nat. Methods. 2015;12:59–60. - PubMed

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