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. 1989 Jun 30;491(1):139-49.
doi: 10.1016/s0378-4347(00)82827-3.

High-performance liquid chromatographic determination of ibuprofen, its metabolites and enantiomers in biological fluids

G Geisslinger  1 , K Dietzel, D Loew, O Schuster, G Rau, G Lachmann, K Brune
Affiliations

High-performance liquid chromatographic determination of ibuprofen, its metabolites and enantiomers in biological fluids

G Geisslinger et al. J Chromatogr. .

Abstract

An isocratic high-performance liquid chromatographic method to determine racemic ibuprofen (assay I) and its major metabolites (assay II) in biological fluids (plasma, urine, bile) using a conventional reversed-phase column is described. A third assay using beta-cyclodextrin as stationary phase (Cyclobond I) for the separation of the ibuprofen enantiomers is also described. A wavelength of 220 nm was used to monitor the substances. The sensitivity of the method was 0.1 microgram/ml for all three assays. The method was demonstrated to be suitable for stereoselective pharmacokinetic studies of ibuprofen in humans and animals.

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