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Comment
. 2017 Apr;101(4):e152-e153.
doi: 10.1097/TP.0000000000001607.

Conformational Variants of HLA-I Antigens on Luminex Single Antigen Beads

Jonathan Visentin  1 , Gwendaline Guidicelli, Jean-Luc Taupin
Affiliations
Comment

Conformational Variants of HLA-I Antigens on Luminex Single Antigen Beads

Jonathan Visentin et al. Transplantation. 2017 Apr.
No abstract available

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Conflict of interest statement

The authors declare no funding or conflicts of interest.

Figures

FIGURE 1.
FIGURE 1.
Reactivity of 3 monoclonal antibodies on nontreated and acid-treated HLA-I LSAB. Nontreated and acid-treated LSAB were incubated with W6/32, anti-β2m (both from Thermo Fisher Scientific, Rockford, IL) and HC-10 (kindly provided by One Lambda) at 10 μg/mL, then were washed and incubated with 200-fold diluted PE-labeled Goat F(ab’)2 fragment anti-mouse IgG (H + L) (Beckman Coulter, Marseille, France). After acid treatment, all beads reached MFI < 1000 with W6/32 and 3 beads still had MFI ≥ 1000 with anti-β2m.
See this image and copyright information in PMC

Comment in

  • The Authors' Reply.
    Ravindranath MH, Jucaud V. Ravindranath MH, et al. Transplantation. 2017 Apr;101(4):e153-e154. doi: 10.1097/TP.0000000000001629. Transplantation. 2017. PMID: 28323776 Free PMC article. No abstract available.

Comment on

References

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