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. 2016 Dec 12;11(12):e0167702.
doi: 10.1371/journal.pone.0167702. eCollection 2016.

Evaluation of 19,460 Wheat Accessions Conserved in the Indian National Genebank to Identify New Sources of Resistance to Rust and Spot Blotch Diseases

Sundeep Kumar  1 , Sunil Archak  1 , R K Tyagi  1 , Jagdish Kumar  2 , Vikas Vk  2 , Sherry R Jacob  1 , Kalyani Srinivasan  1 , J Radhamani  1 , R Parimalan  1 , M Sivaswamy  2 , Sandhya Tyagi  1 , Mamata Yadav  1 , Jyotisna Kumari  1 , Deepali  1 , Sandeep Sharma  1 , Indoo Bhagat  3 , Madhu Meeta  4 , N S Bains  4 , A K Chowdhury  5 , B C Saha  5 , P M Bhattacharya  5 , Jyoti Kumari  1 , M C Singh  1 , O P Gangwar  6 , P Prasad  6 , S C Bharadwaj  6 , Robin Gogoi  7 , J B Sharma  7 , Sandeep Kumar Gm  8 , M S Saharan  9 , Manas Bag  1 , Anirban Roy  1 , T V Prasad  1 , R K Sharma  1 , M Dutta  1 , Indu Sharma  9 , K C Bansal  1
Affiliations

Evaluation of 19,460 Wheat Accessions Conserved in the Indian National Genebank to Identify New Sources of Resistance to Rust and Spot Blotch Diseases

Sundeep Kumar et al. PLoS One. .

Erratum in

Abstract

A comprehensive germplasm evaluation study of wheat accessions conserved in the Indian National Genebank was conducted to identify sources of rust and spot blotch resistance. Genebank accessions comprising three species of wheat-Triticum aestivum, T. durum and T. dicoccum were screened sequentially at multiple disease hotspots, during the 2011-14 crop seasons, carrying only resistant accessions to the next step of evaluation. Wheat accessions which were found to be resistant in the field were then assayed for seedling resistance and profiled using molecular markers. In the primary evaluation, 19,460 accessions were screened at Wellington (Tamil Nadu), a hotspot for wheat rusts. We identified 4925 accessions to be resistant and these were further evaluated at Gurdaspur (Punjab), a hotspot for stripe rust and at Cooch Behar (West Bengal), a hotspot for spot blotch. The second round evaluation identified 498 accessions potentially resistant to multiple rusts and 868 accessions potentially resistant to spot blotch. Evaluation of rust resistant accessions for seedling resistance against seven virulent pathotypes of three rusts under artificial epiphytotic conditions identified 137 accessions potentially resistant to multiple rusts. Molecular analysis to identify different combinations of genetic loci imparting resistance to leaf rust, stem rust, stripe rust and spot blotch using linked molecular markers, identified 45 wheat accessions containing known resistance genes against all three rusts as well as a QTL for spot blotch resistance. The resistant germplasm accessions, particularly against stripe rust, identified in this study can be excellent potential candidates to be employed for breeding resistance into the background of high yielding wheat cultivars through conventional or molecular breeding approaches, and are expected to contribute toward food security at national and global levels.

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Source of wheat germplasm screened in the study.
Numerical details of (A) spatial and (B) temporal augmentation of wheat accessions conserved in the Indian National Genebank that were employed to identify sources of resistance against rusts and spot blotch.
Fig 2
Fig 2. Locations of evaluation experiments of wheat germplasm against rusts and spot blotch.
Primary screening against the three rusts was carried out at Wellington. Subsequent screening for stripe rust resistance was done at Gurdaspur and for spot blotch resistance at Cooch Behar. The seedling resistance assay was carried out at Flowerdale and molecular profiling was done at New Delhi. Base map was generated using DIVA-GIS data (www.diva-gis.org/gdata).
Fig 3
Fig 3. Experimental layout, flow of germplasm and salient results of the study.
Blue filled boxes denote germplasm at various stages of the experiment. Trapezoids denote screening (green for field evaluation and red for lab assay). Figures and tables containing details corresponding to each stage of the flow are mentioned in italics below boxes.
Fig 4
Fig 4. Results of primary screening of wheat germplasm against three rusts.
Stem: Stem rust; Leaf: Leaf rust; Stripe: Stripe rust S: Susceptible; MS: Moderately susceptible; MR: Moderately resistant; R: Resistant.
Fig 5
Fig 5. Amplicon profile of 137 wheat accessions for SSR marker Gwm427 linked to Sr13. M = 100bp ladder.
Fig 6
Fig 6. Diversity among rust resistant genotypes based on profiles of linked molecular markers.
See this image and copyright information in PMC

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