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. 2016 Nov;4(21):438.
doi: 10.21037/atm.2016.08.53.

THP-1 cells as a model for human monocytes

Herbert Bosshart  1 , Michael Heinzelmann  2
Affiliations

THP-1 cells as a model for human monocytes

Herbert Bosshart et al. Ann Transl Med. 2016 Nov.
No abstract available

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Conflict of interest statement

The authors have no conflicts of interest to declare.

Figures

Figure 1
Figure 1
LPS responses in THP-1 cells and primary human monocytes. (A-C) IL-8 production in LPS-stimulated stably transfected THP-1 cells. Cells were added to saline (0 ng/mL LPS) or LPS (1,000, 100, 10 ng/mL) and samples placed on ice (A) or incubated for 2 h (B) or 4 h (C) at 37 °C. IL-8 was measured in supernatants. Depicted are sigmoid dose (x-axis)—response (y-axis) curves of mock-transfected (open circles) and CD14-transfected (closed circles) THP-1 cells. Results from a representative experiment are shown. Note that mock-transfected THP-1 cells accumulate very low amounts of IL-8 after 4 h of incubation with 10 ng/mL LPS; (D) IL-8 production in LPS-stimulated human peripheral blood monocytes. On 3 separate days, venous whole blood from five healthy volunteers (a-e) was collected in ACD Vacutainers and diluted 1:1 with RPMI 1640 medium. Aliquots were added to saline or LPS (10 ng/mL) and incubated for 4 h at 37 °C. IL-8 was measured in supernatants. IL-8 baseline values (saline) above detection level were subtracted from IL-8 values after LPS exposure. Samples from the same individual are represented by identical symbols, with collections that were at least 6 days and no longer than 189 days apart. Note that, over time, different individuals can have similar monocyte counts, yet widely diverging monocyte activities (c,d,e), similar monocyte activities yet widely diverging monocyte counts (a,b,c), or widely diverging monocyte counts and widely diverging monocyte activities (a,e). IL-8, interleukin-8; LPS, lipopolysaccharide; ACD, acid-citrate-dextrose.
See this image and copyright information in PMC

References

    1. Sallusto F, Lanzavecchia A. Monocytes join the dendritic cell family. Cell 2010;143:339-40. 10.1016/j.cell.2010.10.022 - DOI - PubMed
    1. Epelman S, Lavine KJ, Randolph GJ. Origin and functions of tissue macrophages. Immunity 2014;41:21-35. 10.1016/j.immuni.2014.06.013 - DOI - PMC - PubMed
    1. Tsuchiya S, Yamabe M, Yamaguchi Y, et al. Establishment and characterization of a human acute monocytic leukemia cell line (THP-1). Int J Cancer 1980;26:171-6. 10.1002/ijc.2910260208 - DOI - PubMed
    1. Hu ZD, Wei TT, Tang QQ, et al. Gene expression profile of THP-1 cells treated with heat-killed Candida albicans. Ann Transl Med 2016;4:170. 10.21037/atm.2016.05.03 - DOI - PMC - PubMed
    1. Kim JI, Lee CJ, Jin MS, et al. Crystal structure of CD14 and its implications for lipopolysaccharide signaling. J Biol Chem 2005;280:11347-51. 10.1074/jbc.M414607200 - DOI - PubMed