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. 1989 Sep 1;122(2):155-9.
doi: 10.1016/0022-1759(89)90259-7.

Specific immunocytochemical visualization of phosphatidylserine

L Maneta-Peyret  1 , G Freyburger, J J Bessoule, C Cassagne
Affiliations

Specific immunocytochemical visualization of phosphatidylserine

L Maneta-Peyret et al. J Immunol Methods. .

Abstract

In order to determine whether anti-phosphatidylserine antibodies are able to detect phosphatidylserine in situ, an immunocytochemical approach has been developed using human platelets. A strong positive reaction was obtained when anti-phosphatidylserine serum was applied to platelets whereas no reaction was observed with preimmune serum, or with immune serum which had been preincubated with 10(-5) M phosphatidylserine, suggesting that phosphatidylserine was indeed specifically detected by the antibodies.

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