[The application of peroxyoxalate-chemiluminescence in biochemical analysis: determination of oxalate]
- PMID: 2794878
[The application of peroxyoxalate-chemiluminescence in biochemical analysis: determination of oxalate]
Abstract
A new sensitive method for the determination of oxalate is presented. By using the chemiluminescence decay of monoperoxyoxalic acid very low concentrations of oxalate can be determined (up to 100 nmol/l). Oxalate determinations in urine require the precipitation of calcium oxalate, in order to avoid oxalate-unspecific side emissions. This could be avoided by combination with a specific enzymatic partial reaction, which would also permit the determination of low concentrations of other organic species (e.g. citrate, pyruvate, malate).