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. 2017 Jan 2;216(1):21-24.
doi: 10.1083/jcb.201612010. Epub 2016 Dec 12.

Why proteomics is not the new genomics and the future of mass spectrometry in cell biology

Simone Sidoli  1 , Katarzyna Kulej  1   2 , Benjamin A Garcia  3
Affiliations

Why proteomics is not the new genomics and the future of mass spectrometry in cell biology

Simone Sidoli et al. J Cell Biol. .

Abstract

Mass spectrometry (MS) is an essential part of the cell biologist's proteomics toolkit, allowing analyses at molecular and system-wide scales. However, proteomics still lag behind genomics in popularity and ease of use. We discuss key differences between MS-based -omics and other booming -omics technologies and highlight what we view as the future of MS and its role in our increasingly deep understanding of cell biology.

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Figures

Figure 1.
Figure 1.
MS past, present, and future. (A) Number of publications containing the terms genomics, proteomics, or metabolomics in title or abstract (based on PubMed). Each value per year was normalized by the total across all years analyzed. (B) Same representation dating back to 1996. Papers were counted if they contained the term “mass spectrometry” plus the term listed in the legend. (C) Representation of applications of MS-based proteomics studies. Ambient ionization allows for site-specific identification of analytes; cross-linking preserves interactions; ion mobility allows for separation of same-mass analytes based on their cross section; at the end of the pipeline, the mass analyzer determines mass and intensity of analytes.
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