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. 2016 Dec 14;16(4):377-385.

Effects of oncostatin M on cell proliferation and osteogenic differentiation in C3H10T1/2

F Zou  1 , J-C Xu, G-H Wu, L-L Zhou, Q-D Wa, J-Q Peng, X-N Zou
Affiliations

Effects of oncostatin M on cell proliferation and osteogenic differentiation in C3H10T1/2

F Zou et al. J Musculoskelet Neuronal Interact. .

Abstract

Objective: To explore the effects of protein factor Oncostatin M (OSM), a member of the Interleukin-6 (IL-6) family on cell proliferation, osteogenic differentiation and mineralization.

Materials and methods: Basal nutrient solutions of different concentrations of OSM (0, 5, 10, 20, 40, 80 ng/ml) were used. In order to divide embryonic origin between mesenchymal stem cells C3H10T1/2 of in vitro cultured mice, and the effects of in vitro proliferation efficiencies of C3H10T1/2 cells of different concentrations of OSM, the C3H10T1/2 cells were divided into four groups: (1) Basal nutrient solution group (negative control); (2) Osteogenesis induced liquid group (positive control); (3) OSM (20 ng/ml) group; (4) Experimental group (osteogenesis induced liquid + OSM (20 ng/ml)). The expressions levels of relevant osteogenesis and mineralization genes were detected.

Results: OSM had several effects on promoting the proliferation of embryonic origin mesenchymal stem cells C3H10T1/2 with respect to time of exposure as well as concentrations. In the present study, it has been shown that when the concentration of OSM is 20 ng/ml, the effects of promoting proliferation are most obvious. OSM can induce osteogenic differentiation of C3H10T1/2, make the process of osteogenic differentiation in advance, and promote the formation of end-stage calcium deposits and mineralized nodule, and osteogenic differentiation of C3H10T1/2 is finally achieved.

Conclusion: OSM can promote the proliferation of C3H10T1/2, and induce its osteogenic differentiation and end-stage mineralization.

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Conflict of interest statement

The authors have no conflict of interest.

Figures

Figure 1
Figure 1
The effect of OSM on C3H10T1/2 cells proliferation at different time points and different concentrations. OSM treatment at 20 ng/ml significantly increased cell viability. *p<0.05, Compared to 0 ng/ml (OSM); **p<0.01, Compared to 0 ng/ml (OSM).
Figure 2
Figure 2
Expression of ALP activity in the process of osteogenic differentiation.
Figure 3
Figure 3
Calcium quantitative analysis in the process of osteogenic differentiation.
Figure 4
Figure 4
Alizarin red staining during the process of osteogenic differentiation.
Figure 5
Figure 5
Expression levels of genes involved in osteogenesis in the process of osteogenic differentiation detected by RT-PCR.
Figure 6
Figure 6
(A) Zebra Imaging of OC Western-blot; (B) Zebra Imagings of COL-1, OPN and BSP Western-blot.
See this image and copyright information in PMC

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