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Comparative Study
. 1989 Oct 11;17(19):7843-53.
doi: 10.1093/nar/17.19.7843.

Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA

U Edwards  1 , T Rogall, H Blöcker, M Emde, E C Böttger
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Free PMC article
Comparative Study

Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA

U Edwards et al. Nucleic Acids Res. .
Free PMC article

Abstract

Using a set of synthetic oligonucleotides homologous to broadly conserved sequences in-vitro amplification via the polymerase chain reaction followed by direct sequencing results in almost complete nucleotide determination of a gene coding for 16S ribosomal RNA. As a model system the nucleotide sequence of the 16S rRNA gene of M.kansasii was determined and found to be 98.7% homologous to that of M.bovis BCG. This is the first report on a contiguous sequence information of an entire amplified gene spanning 1.5 kb without any subcloning procedures.

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References

    1. J Bacteriol. 1988 Feb;170(2):720-6 - PubMed
    1. Nature. 1988 Aug 4;334(6181):387-8 - PubMed
    1. Nucleic Acids Res. 1988 Nov 11;16(21):10393 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Oct;85(20):7652-6 - PubMed
    1. Science. 1989 Mar 10;243(4896):1360-3 - PubMed

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