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. 1978 Sep;75(9):4544-8.
doi: 10.1073/pnas.75.9.4544.

Isolated microvessels: the blood-brain barrier in vitro

J T Hjelle, J Baird-Lambert, G Cardinale, S Specor, S Udenfriend

Isolated microvessels: the blood-brain barrier in vitro

J T Hjelle et al. Proc Natl Acad Sci U S A. 1978 Sep.

Abstract

Isolated bovine retinal and brain microvessels, exhibiting a patent lumen, were used to study the contribution of the microvasculature to the blood-brain and blood-retina barriers. The diffusion marker, sucrose, was taken up slowly by the isolated microvessels in contrast to leucine, tyrosine, and valine which were taken up at a considerably faster rate. Uptake of leucine was temperature dependent but resistant to inhibition by ouabain and sodium azide. The large neutral amino acids exhibited stereospecificity and cross-competition for uptake by the isolated microvessels. The apparent Kms for uptake for tyrosine, leucine, and valine were III micron,133 micron, and 500 micron, respectively.

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