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. 2016 Nov-Dec;19(6):569-572.
doi: 10.4103/0972-0707.194018.

Comparison of the antimicrobial activity of direct pulp-capping materials: Mineral trioxide aggregate-Angelus and Biodentine

Taha Özyürek  1 , Ebru Özsezer Demiryürek  1
Affiliations

Comparison of the antimicrobial activity of direct pulp-capping materials: Mineral trioxide aggregate-Angelus and Biodentine

Taha Özyürek et al. J Conserv Dent. 2016 Nov-Dec.

Abstract

Purpose: To compare the antimicrobial activity of the tricalcium silicate-based Biodentine (BD) and mineral trioxide aggregate (MTA)-Angelus cement with the aid of agar diffusion test.

Materials and methods: Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Enterococcus faecium were inoculated in the Brucella liquid medium and were incubated at 37°C for 24 h. Thereafter, 100 >μl of the liquid culture of bacteria inoculated in the Mueller-Hinton agar with spread plate technique. Petri plates were dried in room temperature. For every microorganism, 3 petri plates were prepared (12 in total). In the medium, in every petri plate, 2 holes with 5 mm diameter and 2 mm depth were made. Afterward, BD and MTA-Angelus were filled into these holes under aseptic conditions according to the instructions of the manufacturing company. Then, the plates were kept in the incubator at 37°C for 24 h, and the diameters of the inhibition zones were measured with a digital caliper.

Results: Inhibition zones formed by BD against E. coli and S. aureus were significantly larger than the zones formed by MTA-Angelus (P < 0.05). However, the inhibition zones formed by MTA-Angelus against P. aureus and E. faecium were larger than the zones formed by BD (P < 0.05).

Conclusion: Within the limits of the present study, tricalcium silicate-based MTA-Angelus and BD have antimicrobial activity against E. coli, S. aureus, P. aureus, and E. faecium.

Keywords: Agar diffusion test; Biodentine; direct pulp capping; endodontics; mineral trioxide aggregate-Angelus.

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Conflict of interest statement

There are no conflicts of interest.

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