Association between CHFR gene hypermethylation and gastric cancer risk: a meta-analysis

Abstract

Background: The association between the hypermethylation of CHFR gene and gastric cancer risk has been investigated by a number of studies. However, the sample size of the majority of these studies was very small. To get a more a convincing conclusion, here we performed a meta-analysis of the previously published studies to assess the association between CHFR methylation and the risk of gastric cancer.

Methods: Eligible studies were identified by searching the MEDLINE/PubMed, Embase, and Web of Science databases before May 2016 without any language restriction. The strength of the association was estimated by odds ratio with its 95% confidence interval (CI).

Results: Totally 1,399 samples, including 758 gastric cancer cases and 641 controls, from 13 studies were included in the present meta-analysis. Compared with non-cancer controls, the pooled OR of CHFR methylation in gastric cancer patients was 9.08 (95% CI: 6.40-12.88, P<0.001), suggesting that the methylation of CHFR was significantly associated with increased risk of gastric cancer. Similar results were observed when subgroup analyses were performed stratified by country, ethnicity, and methylation testing methods.

Conclusion: Our meta-analysis showed a strong positive correlation between CHFR methylation and risk of gastric cancer, suggesting that CHFR methylation might be a promising biomarker for the diagnosis of gastric cancer.

Keywords: CHFR; gastric cancer; methylation; risk; tumor suppressor gene.

Figure 1

Forest plots of the association between CHFR methylation and gastric cancer risk. Abbreviations: CI, confidence interval; OR, odds ratio.

Figure 2

Begg’s funnel plot of publication biases. Each point represents a separate study. (A) Begg’s funnel plot of publication bias test. (B) Begg’s funnel plot of publication bias test after trim and fill method. Abbreviations: s.e., standard error; OR, odds ratio.