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Case Reports
. 2016 Dec 13:11:89.
doi: 10.1186/s40793-016-0211-5. eCollection 2016.

Complete genome sequence of new bacteriophage phiE142, which causes simultaneously lysis of multidrug-resistant Escherichia coli O157:H7 and Salmonella enterica

Luis Amarillas  1 , Cristobal Chaidez  2 , Arturo González-Robles  3 , Josefina León-Félix  4
Affiliations
Case Reports

Complete genome sequence of new bacteriophage phiE142, which causes simultaneously lysis of multidrug-resistant Escherichia coli O157:H7 and Salmonella enterica

Luis Amarillas et al. Stand Genomic Sci. .

Abstract

The emergence of antibiotic-resistant foodborne bacteria is a global health problem that requires immediate attention. Bacteriophages are a promising biotechnological alternative approach against bacterial pathogens. However, a detailed analysis of phage genomes is essential to assess the safety of the phages prior to their use as biocontrol agents. Therefore, here we report the complete genome sequence of bacteriophage phiE142, which is able to lyse Salmonella and multidrug-resistant Escherichia coli O157:H7 strains. Bacteriophage phiE142 belongs to the Myoviridae family due to the presence of long non-flexible tail and icosahedral head. The genome is composed of 121,442 bp and contains 194 ORFs, and 2 tRNAs. Furthermore, the phiE142 genome does not contain any genes coding for food-borne allergens, antibiotics resistance, virulence factors, or associated with lysogenic conversion. The bacteriophage phiE142 is characterized by broad host range and compelling genetic attributes making them potential candidates as a biocontrol agent.

Keywords: Enterobacteriaceae bacteriophage; Genome sequence; Potential biocontrol agent; Short genome report; phiE142.

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Figures

Fig. 1
Fig. 1
Transmission electron microscopy (TEM) of bacteriophage phiE142, which exhibits an icosahedral head, long and non-flexible tail. This morphology corresponds to the Myoviridae family
Fig. 2
Fig. 2
Phylogenetic tree based on the predicted amino acid sequences of the DNA polymerase of 17 bacteriophages. Phylogenetic tree was performed in MEGA 6.0 version by the neighbor joining method with the Jukes Cantor model. The percentages of bootstrap samples (of 1,000) are indicated for each internal branch. The scale bar indicates the proportion of substitutions per site
Fig. 3
Fig. 3
Diagram of bacteriophage genome. The arrows indicated open reading frame (ORF), the orientation of which shows the direction of transcription. Putative genes are colored according to the predicted functions of their products: Phage structural proteins (blue arrows), DNA regulation module (green arrows), packaging module (pink arrows), host lysis proteins (red arrows), and hypothetical proteins (gray arrows)
See this image and copyright information in PMC

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