Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Dec 22:6:39493.
doi: 10.1038/srep39493.

Gene expression differences in Longissimus muscle of Nelore steers genetically divergent for residual feed intake

Polyana C Tizioto  1   2 , Luiz L Coutinho  3 , Priscila S N Oliveira  1 , Aline S M Cesar  3 , Wellison J S Diniz  4 , Andressa O Lima  4 , Marina I Rocha  4 , Jared E Decker  2   5 , Robert D Schnabel  2   5 , Gerson B Mourão  3 , Rymer R Tullio  1 , Adhemar Zerlotini  6 , Jeremy F Taylor  2 , Luciana C A Regitano  1
Affiliations

Gene expression differences in Longissimus muscle of Nelore steers genetically divergent for residual feed intake

Polyana C Tizioto et al. Sci Rep. .

Erratum in

Abstract

Residual feed intake (RFI), a measure of feed efficiency (FE), is defined as the difference between the observed and the predictable feed intake considering size and growth of the animal. It is extremely important to beef production systems due to its impact on the allocation of land areas to alternative agricultural production, animal methane emissions, food demand and cost of production. Global differential gene expression analysis between high and low RFI groups (HRFI and LRFI: less and more efficient, respectively) revealed 73 differentially expressed (DE) annotated genes in Longissimus thoracis (LT) muscle of Nelore steers. These genes are involved in the overrepresented pathways Metabolism of Xenobiotics by Cytochrome P450 and Butanoate and Tryptophan Metabolism. Among the DE transcripts were several proteins related to mitochondrial function and the metabolism of lipids. Our findings indicate that observed gene expression differences are primarily related to metabolic processes underlying oxidative stress. Genes involved in the metabolism of xenobiotics and antioxidant mechanisms were primarily down-regulated, while genes responsible for lipid oxidation and ketogenesis were up-regulated in HRFI group. By using LT muscle, this study reinforces our previous findings using liver tissue and reveals new genes and likely tissue-specific regulators playing key-roles in these processes.

PubMed Disclaimer

Figures

Figure 1
Figure 1. Network integration of differentially expressed genes between the efficient (LRFI) and inefficient (HRFI) groups.
Genes presented as black circles were differentially expressed (DE) between the efficient (LRFI) and inefficient (HRFI) groups. Genes presented in grey interact with the DE genes. Circles highlighted in yellow are transcription factors. Arrows in pink, green, blue and red represent co-expression relationships, genetic interactions, co-localizations and physical interactions, respectively.
Figure 2
Figure 2. Network integration of the EGR1 gene and other differentially expressed genes.
Genes presented as black circles were differentially expressed (DE) between the efficient (LRFI) and inefficient (HRFI) groups. Genes presented in grey interact with the DE genes. Arrows presented in pink, green, blue and red represent co-expression relationships, genetic interactions, co-localizations and physical interactions, respectively.
Figure 3
Figure 3. Venn diagram showing overlaps between differentially expressed genes found for residual feed intake (RFI), average daily gain (ADG) and dry matter intake (DMI).
See this image and copyright information in PMC

References

    1. Archer J. A., Richardson E. C., Herd R. M. & Arthur P. F. Potential for selection to improve efficiency of feed use in beef cattle: A review. Austr J Agric Res 50, 147–161 (1999).
    1. Arthur P. F. et al.. Genetic and phenotypic variance and covariance components for feed intake, feed efficiency, and other postweaning traits in Angus cattle. J Anim Sci 79, 2805–2811 (2001). - PubMed
    1. Schenkel F. S., Miller S. P. & Wilton J. W. Genetic parameters and breed differences for feed efficiency, growth, and body composition traits of young beef bulls. Can J Anim Sci 84, 177–185 (2004).
    1. Spurlock D. M., Dekker J. C. M., Fernando R., Koltes D. A. & Wolc A. Genetic parameters for energy balance, feed efficiency, and related traits in Holstein cattle. J Dairy Sci. 95, 5393–5402 (2012). - PubMed
    1. Nkrumah J. D. et al.. Genetic and phenotypic relationships of feed intake and measures of efficiency with growth and carcass merit of beef cattle. J Anim Sci. 85, 2711–2720 (2007). - PubMed

Publication types

LinkOut - more resources