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. 2016 Dec 22;11(12):e0168442.
doi: 10.1371/journal.pone.0168442. eCollection 2016.

Evaluation of Bt Corn with Pyramided Genes on Efficacy and Insect Resistance Management for the Asian Corn Borer in China

Fan Jiang  1 , Tiantao Zhang  1 , Shuxiong Bai  1 , Zhenying Wang  1 , Kanglai He  1
Affiliations

Evaluation of Bt Corn with Pyramided Genes on Efficacy and Insect Resistance Management for the Asian Corn Borer in China

Fan Jiang et al. PLoS One. .

Abstract

A Bt corn hybrid (AcIe) with two Bt genes (cry1Ie and cry1Ac) was derived by breeding stack from line expressing Cry1Ie and a line expressing Cry1Ac. Efficacy of this pyramided Bt corn hybrid against the Asian corn borer (ACB), Ostrinia furnacalis, was evaluated. We conducted laboratory bioassays using susceptible and resistant ACB strains fed on artificial diet or fresh plant tissues. We also conducted field trials with artificial infestations of ACB neonates at the V6 and silk stages. The toxin-diet bioassay data indicated that mixtures of Cry1Ac and Cry1Ie proteins had synergistic insecticidal efficacy. The plant tissue bioassay data indicated that Bt corn hybrids expressing either a single toxin (Cry1Ac or Cry1Ie) or two toxins had high efficacy against susceptible ACB. Damage ratings in the field trials indicated that the Bt corn hybrids could effectively protect against 1st and the 2nd generation ACB in China. The hybrid line with two Bt genes showed a higher efficacy against ACB larvae resistant to Cry1Ac or CryIe than the hybrid containing one Bt gene, and the two gene hybrid would have increased potential for managing or delaying the evolution of ACB resistance to Bt corn plants.

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Conflict of interest statement

The authors have declared that no competing interests exist.

References

    1. Zhou DR, He KL, Wang ZY, Ye ZH, Wen LP, Gao YX, et al. Asian corn borer and its integrated management (In Chinese). Beijing, China: Golden Shield Press; 1995.
    1. Xu L, Wang Z, Zhang J, He K, Ferry N, Gatehouse AMR. Cross-resistance of Cry1Ab-selected Asian corn borer to other Cry toxins. J Appl Entomol. 2010; 134(5): 429–38.
    1. Huang FN, Higgins RA, Buschman LL. Baseline susceptibility and changes in susceptibility to Bacillus thuringiensis subsp. kurstaki under selection pressure in European corn borer (Lepidoptera: Pyralidae). J Econ Entomol. 1997; 90(5): 1137–43.
    1. Bolin PC, Hutchison WD, Andow DA. Long-term selection for resistance to Bacillus thuringiensis Cry1Ac endotoxin in a minnesota population of European corn borer (Lepidoptera: Crambidae). J Econ Entomol. 1999; 92(5): 1121–30.
    1. Chaufaux J, Seguin M, Swanson JJ, Bourguet D, Siegfried BD. Chronic exposure of the European corn borer (Lepidoptera: Crambidae) to Cry1Ab Bacillus thuringiensis toxin. J Econ Entomol. 2001; 94(6): 1564–70. - PubMed

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