Altered electrical potential profile of human reabsorptive sweat duct cells in cystic fibrosis

Abstract

The electrophysiological properties of reabsorptive sweat duct (RSD) cells from normal and cystic fibrosis (CF) subjects were studied using intracellular microelectrodes. The apical membrane potential (Va) of CF duct cells was reversed in "polarity" (+28.0 +/- 2.4 mV, n = 46) compared with normal duct cells (-24.9 +/- 0.4 mV, n = 145), and the basolateral membrane potential (Vb) of CF cells was hyperpolarized significantly (-50.1 +/- 1.2 mV, n = 46) in comparison to normal cells (-34.6 +/- 0.4 mV, n = 145). The substitution of the impermeant anion gluconate for Cl- in the lumen of the normal duct depolarized Va from -24.9 +/- 1.1 to 8.9 +/- 3.1 mV (n = 18) and hyperpolarized Vb from -34.3 +/- 1.1 to -55.6 +/- 3.7 mV (n = 18), which mimicked the cell electrical potential profile of CF ducts even in the presence of Cl-. Cl- substitution in the bath depolarized Vb of normal ducts by 22.5 +/- 2.6 mV (n = 24), while hyperpolarizing Va by -3.4 +/- 1.6 mV (n = 24). The response of the electrical profiles of CF cells to Cl- substitution in either the lumen or the bath was significantly reduced compared with normal cells. The effect of the Na+ conductance blocker amiloride (10(-4) M) on Vb was not significantly different in CF (delta Vb = -26.4 +/- 3.8 mV, n = 9) vs. normal (delta Vb = -27.6 +/- 2.5 mV, n = 30) cells.(ABSTRACT TRUNCATED AT 250 WORDS)