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. 2017 Feb:133:52-54.
doi: 10.1016/j.mimet.2016.12.018. Epub 2016 Dec 24.

Stringent control of FLP recombinase in Escherichia coli

Steven D Bowden  1 , Nagendra P Palani  2 , Igor G L Libourel  3
Affiliations

Stringent control of FLP recombinase in Escherichia coli

Steven D Bowden et al. J Microbiol Methods. 2017 Feb.

Abstract

Site specific recombinases are invaluable tools in molecular biology, and are emerging as powerful recorders of cellular events in synthetic biology. We have developed a stringently controlled FLP recombinase system in Escherichia coli using an arabinose inducible promoter combined with a weak ribosome binding site.

Keywords: Escherichia coli, arabinose; Flp recombinase; Gene expression; Repression.

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