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. 2016 Nov 28:5:182.
doi: 10.4103/2277-9175.190985. eCollection 2016.

Preparation and evaluation of a hair wax containing propolis and Eruca sativa seed oil for hair growth

Mohammad-Ali Shatalebi  1 , Leila Safaeian  2 , Azar Baradaran  3 , Mozhde Alamdarian  1
Affiliations

Preparation and evaluation of a hair wax containing propolis and Eruca sativa seed oil for hair growth

Mohammad-Ali Shatalebi et al. Adv Biomed Res. .

Abstract

Background: Hair growth as a key consumer objective has important role in the hair care products researches. This study was aimed to investigate the effect of a hair wax containing propolis, a resinous mixture produced by honeybees in Eruca sativa seed oil base on hair growth.

Materials and methods: The hair wax was designed and formulated compared with marketed brand hair wax and evaluated for pharmaceutical parameters including pH, homogeneity, consistency, spread ability, in vitro drug release, and stability. After selection of the best formulation containing 10% ethanolic extract of propolis and 10% E. sativa seed oil, the hair growth potential was evaluated by application of 1 g hair wax daily on 4 cm2 area of dorsal side of Wistar rats and compared with controls and standard medication (1 ml of 2% minoxidil). After 30 days treatment, the length and weight of hairs and percentage of hair follicles in different phases of growth in skin biopsies were assessed.

Results: The selected hair wax formulation was stable and easy to wash. The formulation significantly increased hair length on 10th, 20th, and 30th day compared control group (5.8 ± 0.3 vs. 2.6 ± 0.4, 11.4 ± 0.6 vs. 5.8 ± 0.4, and 17.5 ± 0.5 vs. 12.7 ± 0.4 mm, respectively) and also the weight of newly grown hairs on 30th day (0.056 ± 0.006 vs. 0.043 ± 0.005). It improved hair follicles percentages in anagen phase without any sensitivity reaction.

Conclusions: The results of this study suggest that the formulated hair wax containing of propolis and E. sativa seed oil could have significant effect on promoting hair growth.

Keywords: Eruca sativa Mill.; hair growth; hair wax; propolis.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Percentage of cumulative drug release of selected formulation (F6) in Franz diffusion cell through a cellulose acetate membrane at 37°C during 6 h. Values are expressed as mean ± standard error of mean
Figure 2
Figure 2
Effects of various treatments on hair length of Wistar rat at different times. Values are expressed as mean ± standard error of mean **P < 0.01 and ***P < 0.001 versus control group
Figure 3
Figure 3
Effects of various treatments on hair weight of Wistar rat. Values are expressed as mean ± standard error of mean *P < 0.05 and **P < 0.01 versus control group
Figure 4
Figure 4
Skin histology sections of Wistar rats; (a) control, (b) minoxidil 2%, (c) hair wax base containing Eruca sativa seed oil, (d) selected formulation containing propolis and Eruca sativa seed oil, (e) hair wax base containing liquid paraffin
Figure 5
Figure 5
Effects of various treatments on the percentage of hair follicles in the anagen and telogen phases. Values are expressed as mean ± standard error of mean *P < 0.01 and **P < 0.001 versus control group
Figure 6
Figure 6
Effects of various treatments on hair growth of Wistar rats on the 1st day (a) and after 30 days; (b) control, (c) minoxidil 2%, (d) hair wax base containing Eruca sativa seed oil, (e) Selected formulation containing propolis and Eruca sativa seed oil, (f) hair wax base containing liquid paraffin
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