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. 2016 Dec 28;11(12):e0168728.
doi: 10.1371/journal.pone.0168728. eCollection 2016.

Contribution of 1p, 19q, 9p and 10q Automated Analysis by FISH to the Diagnosis and Prognosis of Oligodendroglial Tumors According to WHO 2016 Guidelines

Karine Michaud  1 , Marie de Tayrac  2 , Myreille D'Astous  1 , Céline Duval  3 , Claudie Paquet  3 , Oumar Samassekou  3 , Peter Vincent Gould  3 , Stéphan Saikali  3
Affiliations

Contribution of 1p, 19q, 9p and 10q Automated Analysis by FISH to the Diagnosis and Prognosis of Oligodendroglial Tumors According to WHO 2016 Guidelines

Karine Michaud et al. PLoS One. .

Abstract

Objective: To study the feasibility and the diagnostic and prognostic interest of automated analysis of 1p, 19q, 9p and 10q status by FISH technique in oligodendroglial tumors.

Methods: We analyzed a retrospective series of 33 consecutive gliomas with oligodendroglial histology (originally diagnosed as 24 oligodendrogliomas and 9 oligoastrocytomas). For all cases, automated FISH analysis of 1p, 19q, 9p and 10q status were performed and compared to clinical and histological data, ATRX, IDH1R132H and alpha-internexin status (studied by immunohistochemistry) and overall survival (OS). Manual analysis of 9p and 10q status were also performed and compared to automated analysis to verify the concordance of the two methods.

Results: The 33 gliomas were reclassified into 13 low-grade oligodendrogliomas (OII), 10 anaplastic oligodendrogliomas (OIII), 3 diffuse astrocytomas (AII), 3 anaplastic astrocytomas (AIII) and 4 glioblastomas (GBM) according to the WHO 2016 histological criteria. The 1p and/or 19q imbalanced status were restricted to astrocytomas with no correlation to their grade or their OS. Chromosome 9p deletion was restricted to OIII (70%) and GBM (100%) and was correlated with a shorter OS in the total cohort (p = 0.0007), the oligodendroglioma cohort (p = 0.03) and the astrocytoma cohort (p = 0.001). Concordance between 9p manual and automated analysis was satisfactory (81%, κ = 0.69). Chromosome 10q deletion was restricted to GBMs (50%) and was correlated with a poor OS in both the total cohort (p = 0.003) and the astrocytoma (AS) cohort (p = 0.04). Concordance between manual and automated analysis was satisfactory (79%, κ = 0.62).

Conclusion: Automated analysis of 1p, 19q, 9p and 10q status by FISH is a reliable technique which allows for refined classification of oligodendroglial tumors. 1p and/or 19q imbalanced status is evidence of astrocytic differentiation. 9p deletion is found in high grade oligodendrogliomas and astrocytomas with a poor OS. 10q is related to GBM status and a poor OS.

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1
Representative hybridizations from oligodendrogliomas (a-d) and glioblastoma (e). a: 9 p normal status with 2 Green control and 2 Red target signals (2R/2G) in majority of tumoral cells. b: 9p deletion with 1R/2G signal in most nuclei. c: 9p imbalance with an increased copy number of red and green signal. d: codeletion of 9p (red) and 9q (green) consistent with a whole chromosome 9 deletion (monosomy 9). e: deletion of PTEN 1R/2G consistent with 10q deletion. Note the presence of frequent background fluorescent artifacts.
Fig 2
Fig 2. OS curves according to histological and chromosomal status.
OS by histological grading (a), chromosome 9p deletion in oligodendrogliomas (b) and astrocytomas (c) and chromosome 10q deletion in astrocytomas.
See this image and copyright information in PMC

References

    1. Louis DN, Perry A, Reifenberger G, von Deimling A, Figarella-Branger D, Cavenee WK et al. The 2016 World Health Organization Classification of tumors of the central nervous system: a summary. Acta Neuropathol. 2016;5: 429–435. - PubMed
    1. Sahm F, Reuss D, Koelsche C, Capper D, Schittenhelm J, Heim S, et al. Farewell to oligoastrocytoma: in situ molecular genetics favor classification as either oligodendroglioma or astrocytoma. Acta Neuropathol. 2014;128: 551–559 10.1007/s00401-014-1326-7 - DOI - PubMed
    1. Quaddoumi I, Orisme W, Wen J, Santiago T, Gupta K, Dalton JD et al. Genetic alterations in uncommon low grade neuroepithelial tumors: BRAF, FGFR1, and MYB mutations occur at high frequency and align with morphology. Acta Neuropathol. 2016;131: 833–845. 10.1007/s00401-016-1539-z - DOI - PMC - PubMed
    1. Cairncross G, Jenkins R. Gliomas with 1p/19q codeletion: a.k.a. oligodendroglioma. Cancer J. 2008;14: 352–357. 10.1097/PPO.0b013e31818d8178 - DOI - PubMed
    1. Cairncross G, Wang M, Shaw E, Jenkins R, Brachman D, Buckner J, et al. Phase III trial of chemoradiotherapy for anaplastic oligodendroglioma: long-term results of RTOG 9402. J Clin Oncol Off J Am Soc Clin Oncol. 2013;31: 337–343. - PMC - PubMed